Projects per year
Abstract
We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.
| Original language | English |
|---|---|
| Article number | 050901 |
| Number of pages | 4 |
| Journal | Physical Review E: Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics |
| Volume | 79 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 28 May 2009 |
Keywords
- fluorescence
- intramolecular mechanics
- macromolecules
- molecular biophysics
- proteins
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Dive into the research topics of 'Protein fluorescence decay: a gamma function description of thermally induced interconversion of amino acid rotamers'. Together they form a unique fingerprint.Projects
- 1 Finished
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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D. (Principal Investigator), Dawson, M. (Co-investigator), Faulds, K. (Co-investigator), Girkin, J. (Co-investigator), Graham, D. (Co-investigator), Martin, R. (Co-investigator), O'Donnell, K. (Co-investigator), Rolinski, O. (Co-investigator), Smith, W. (Co-investigator) & Wynne, K. (Co-investigator)
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research