Protein fluorescence decay: a gamma function description of thermally induced interconversion of amino acid rotamers

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Abstract

We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.
LanguageEnglish
Article number050901
Number of pages4
JournalPhysical Review E: Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
Volume79
Issue number5
DOIs
Publication statusPublished - 28 May 2009

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gamma function
Gamma function
albumins
serums
Fluorescence
amino acids
Amino Acids
Decay
oxazole
proteins
Protein
fluorescence
tryptophan
decay
Temperature Dependence
Kinetics
Monitoring
Distinct
temperature dependence
kinetics

Keywords

  • fluorescence
  • intramolecular mechanics
  • macromolecules
  • molecular biophysics
  • proteins

Cite this

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title = "Protein fluorescence decay: a gamma function description of thermally induced interconversion of amino acid rotamers",
abstract = "We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.",
keywords = "fluorescence, intramolecular mechanics, macromolecules, molecular biophysics, proteins",
author = "O.J. Rolinski and K. Scobie and D.J.S. Birch",
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doi = "10.1103/PhysRevE.79.050901",
language = "English",
volume = "79",
journal = "Physical Review E",
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AU - Scobie, K.

AU - Birch, D.J.S.

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N2 - We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.

AB - We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.

KW - fluorescence

KW - intramolecular mechanics

KW - macromolecules

KW - molecular biophysics

KW - proteins

U2 - 10.1103/PhysRevE.79.050901

DO - 10.1103/PhysRevE.79.050901

M3 - Article

VL - 79

JO - Physical Review E

JF - Physical Review E

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