Protein fluorescence decay: a gamma function description of thermally induced interconversion of amino acid rotamers

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We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.
Original languageEnglish
Article number050901
Number of pages4
JournalPhysical Review E: Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
Issue number5
Publication statusPublished - 28 May 2009


  • fluorescence
  • intramolecular mechanics
  • macromolecules
  • molecular biophysics
  • proteins

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