Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

Li Hung C Chung; David J S Birch; Vladislav Vyshemirsky; Angelo Bella; Maxim G Ryadnov; Olaf J Rolinski

doi:10.1088/2050-6120/ab1985

Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

Li Hung C Chung, David J S Birch, Vladislav Vyshemirsky, Angelo Bella, Maxim G Ryadnov, Olaf J Rolinski

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Abstract

The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.

Original language	English
Article number	035003
Number of pages	8
Journal	Methods and Applications in Fluorescence
Volume	7
Issue number	3
DOIs	https://doi.org/10.1088/2050-6120/ab1985
Publication status	Published - 16 May 2019

Keywords

protein fibrillogenesis
fluorescence intensity decay
time-resolved emission spectra
glycation

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10.1088/2050-6120/ab1985

Chung-etal-MAF-2019-Protein-fibrillogenesis-model-tracked-by-its-intrinsic-time-resolved-emission-spectraAccepted author manuscript, 2.61 MB

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title = "Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra",

abstract = "The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.",

keywords = "protein fibrillogenesis, fluorescence intensity decay, time-resolved emission spectra, glycation",

author = "Chung, {Li Hung C} and Birch, {David J S} and Vladislav Vyshemirsky and Angelo Bella and Ryadnov, {Maxim G} and Rolinski, {Olaf J}",

year = "2019",

month = may,

day = "16",

doi = "10.1088/2050-6120/ab1985",

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T1 - Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

AU - Chung, Li Hung C

AU - Birch, David J S

AU - Vyshemirsky, Vladislav

AU - Bella, Angelo

AU - Ryadnov, Maxim G

AU - Rolinski, Olaf J

PY - 2019/5/16

Y1 - 2019/5/16

N2 - The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.

AB - The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.

KW - protein fibrillogenesis

KW - fluorescence intensity decay

KW - time-resolved emission spectra

KW - glycation

UR - https://iopscience.iop.org/journal/2050-6120

U2 - 10.1088/2050-6120/ab1985

DO - 10.1088/2050-6120/ab1985

M3 - Article

SN - 2050-6120

VL - 7

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 3

M1 - 035003

ER -

Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

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Keywords

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