Protein enrichment of corn cob heteroxylan waste slurry by thermophilic aerobic digestion using Bacillusstearothermophilus

J.O. Ugwuanyi, L.M. Harvey, B. McNeil

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Thermophilic aerobic digestion (TAD) of heteroxylan waste was implemented at waste load of 30 g L−1 with mineral nitrogen supplementation to study effect of the process on waste degradation, protein accretion and quality. Digestions were carried out at 45 50, 55, 60 and 65 °C using Bacillus stearothermophilus in a CSTR under batch conditions at 1.0 vvm aeration rate, pH 7.0 for a maximum of 120 h. Amylase and xylanase activities appeared rapidly in the digest, while basal protease activity appeared early in the digestion and increased towards end of the processes. Highest degradation of volatile suspended solid, hemicellulose and fibre occurred at 55 °C while highest degradation of total suspended solid occurred at 60 °C. Highest protein accretion (258.8%) and assimilation of mineral nitrogen and soluble protein occurred at 55 °C. The % content of amino acids of digest crude protein increased relative to raw waste and with digestion temperature. Quality of digest protein was comparable to the FAO standard for feed use. TAD has potentials for use in the protein enrichment of waste.
LanguageEnglish
Pages6974-6985
Number of pages12
JournalBioresource Technology
Volume99
Issue number15
DOIs
Publication statusPublished - Oct 2008

Fingerprint

slurry
digestion
maize
Proteins
protein
Degradation
degradation
Minerals
Nitrogen
accretion
Amylases
nitrogen
Food and Agricultural Organization
Bacilli
mineral
aeration
Amino acids
Peptide Hydrolases
amino acid
Amino Acids

Keywords

  • thermophilic aerobic digestion (TAD)
  • protein enrichment
  • protein quality
  • heteroxylan waste slurry
  • animal feed

Cite this

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abstract = "Thermophilic aerobic digestion (TAD) of heteroxylan waste was implemented at waste load of 30 g L−1 with mineral nitrogen supplementation to study effect of the process on waste degradation, protein accretion and quality. Digestions were carried out at 45 50, 55, 60 and 65 °C using Bacillus stearothermophilus in a CSTR under batch conditions at 1.0 vvm aeration rate, pH 7.0 for a maximum of 120 h. Amylase and xylanase activities appeared rapidly in the digest, while basal protease activity appeared early in the digestion and increased towards end of the processes. Highest degradation of volatile suspended solid, hemicellulose and fibre occurred at 55 °C while highest degradation of total suspended solid occurred at 60 °C. Highest protein accretion (258.8{\%}) and assimilation of mineral nitrogen and soluble protein occurred at 55 °C. The {\%} content of amino acids of digest crude protein increased relative to raw waste and with digestion temperature. Quality of digest protein was comparable to the FAO standard for feed use. TAD has potentials for use in the protein enrichment of waste.",
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Protein enrichment of corn cob heteroxylan waste slurry by thermophilic aerobic digestion using Bacillusstearothermophilus. / Ugwuanyi, J.O.; Harvey, L.M.; McNeil, B.

In: Bioresource Technology, Vol. 99, No. 15, 10.2008, p. 6974-6985.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein enrichment of corn cob heteroxylan waste slurry by thermophilic aerobic digestion using Bacillusstearothermophilus

AU - Ugwuanyi, J.O.

AU - Harvey, L.M.

AU - McNeil, B.

PY - 2008/10

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AB - Thermophilic aerobic digestion (TAD) of heteroxylan waste was implemented at waste load of 30 g L−1 with mineral nitrogen supplementation to study effect of the process on waste degradation, protein accretion and quality. Digestions were carried out at 45 50, 55, 60 and 65 °C using Bacillus stearothermophilus in a CSTR under batch conditions at 1.0 vvm aeration rate, pH 7.0 for a maximum of 120 h. Amylase and xylanase activities appeared rapidly in the digest, while basal protease activity appeared early in the digestion and increased towards end of the processes. Highest degradation of volatile suspended solid, hemicellulose and fibre occurred at 55 °C while highest degradation of total suspended solid occurred at 60 °C. Highest protein accretion (258.8%) and assimilation of mineral nitrogen and soluble protein occurred at 55 °C. The % content of amino acids of digest crude protein increased relative to raw waste and with digestion temperature. Quality of digest protein was comparable to the FAO standard for feed use. TAD has potentials for use in the protein enrichment of waste.

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