Protein coated microcrystals formulated with model antigens and modified with calcium phosphate exhibit enhanced phagocytosis and immunogenicity

Sarah Jones, Catpagavalli Asokanathan, Dorota Kmiec, June Irvine, Roland Fleck, Dorothy Xing, Barry Moore, Roger Parton, John Coote

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Abstract

Protein-coated microcrystals (PCMCs) were investigated as potential vaccine formulations for a range of model antigens. Presentation of antigens as PCMCs increased the antigen-specific IgG responses for all antigens tested, compared to soluble antigens. When compared to conventional aluminium-adjuvanted formulations, PCMCs modified with calcium phosphate (CaP) showed enhanced antigen-specific IgG responses and a decreased antigen-specific IgG1:IgG2a ratio, indicating the induction of a more balanced Th1/Th2 response. The rate of antigen release from CaP PCMCs, in vitro, decreased strongly with increasing CaP loading but their immunogenicity in vivo was not significantly different, suggesting the adjuvanticity was not due to a depot effect. Notably, it was found that CaP modification enhanced the phagocytosis of fluorescent antigen-PCMC particles by J774.2 murine monocyte/macrophage cells compared to soluble antigen or soluble PCMCs. Thus, CaP PCMCs may provide an alternative to conventional aluminium-based acellular vaccines to provide a more balanced Th1/Th2 immune response.
Original languageEnglish
Pages (from-to)4234-4242
Number of pages9
JournalVaccine
Volume32
Issue number33
Early online date10 Oct 2013
DOIs
Publication statusPublished - 16 Jul 2014

Keywords

  • calcium phosphate
  • microparticles
  • phagocytosis
  • adjuvant

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