The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.
- Soluble polymeric support
- safety catch linker
- enzymatic- synthesis
Ulijn, R. V., Baragana, B., Halling, P. J., & Flitsch, S. L. (2002). Protease-catalyzed peptide synthesis on solid support. Journal of the American Chemical Society, 124(37), 10988-10989. https://doi.org/10.1021/ja026912d