Properties of the cellular prion protein expressed in Xenopus oocytes

John G. Connolly, Roth J. Tate, Neil F. McLennan, Debbie Brown, Glenn C. Telling, Jan Fraser, Mark W. Head

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The cellular prion protein (PrPC) from different species can be reproducibly expressed in Xenopus oocytes following injection of in vitro transcribed mRNAs. The level of PrPC accumulation increases with the amount of RNA injected and with the duration of incubation. PrPC expressed in oocytes is similar in size and abundance to PrPC: protein in mouse brain and > 100 ng of PrPC is expressed per oocyte allowing complete experiments to be carried out in single living cells. The protein is glycosylated, fully protease sensitive and expressed on the cell surface. Xenopus oocytes therefore provide a useful model system for the study of prion proteins and their associated disease processes.
Original languageEnglish
Pages (from-to)1229-1233
Number of pages4
JournalNeuroReport
Volume13
Issue number9
Publication statusPublished - 2002

Keywords

  • confocal
  • hamster
  • model system
  • mouse
  • prion protein
  • western
  • Xenopus oocyte

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