Abstract
Language | English |
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Title of host publication | Toxoplasmosis |
Editors | Judith E. Smith |
Place of Publication | Berlin |
Publisher | Springer-Verlag |
Pages | 217-229 |
Number of pages | 13 |
Volume | H78 |
ISBN (Print) | 9783642785610 |
DOIs | |
Publication status | Published - 1993 |
Publication series
Name | Nato ASI Subseries H: |
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Publisher | Springer-Verlag |
Volume | 78 |
ISSN (Print) | 1010-8793 |
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Keywords
- toxoplasmosis
- antigens
Cite this
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Progress towards the development of a vaccine against congenital toxoplasmosis : identification of protective antigens and the selection of appropriate adjuvants. / Alexander, J.; Roberts, C. W.; Brewer, J. M.
Toxoplasmosis. ed. / Judith E. Smith. Vol. H78 Berlin : Springer-Verlag, 1993. p. 217-229 (Nato ASI Subseries H:; Vol. 78).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Progress towards the development of a vaccine against congenital toxoplasmosis
T2 - identification of protective antigens and the selection of appropriate adjuvants
AU - Alexander, J.
AU - Roberts, C. W.
AU - Brewer, J. M.
PY - 1993
Y1 - 1993
N2 - We have recently established that vertical disease transmission from the mother to the foetus, as in humans and sheep, only occurs in BALB/c mice infected with Toxoplasma gondii for first time during pregnancy. Thus a previous infection generally gives life long immunity, as ideally, would an effective vaccine. Of three major fractions harvested from RH strain tachyzoites (membrane, soluble and excretory/secretory) the soluble fraction was found the most effective in reducing mortality and cyst burdens in adult mice. Further studies indicated that the choice of adjuvant could enhance this effect. Entrapment within non-ionic surfactant vesicles (NISV), but not Freund’s Complete Adjuvant, significantly enhanced the protection afforded by soluble antigen to adult mice as well as greatly increasing the T cell specific proliferative response, IFN-γ production and antibody levels. NISV are safe, stable, and inexpensive and promote TH1 and CD8+ lymphocyte activation. In BALB/c dams vaccinated with NISV entrapped soluble antigen and infected on day 12 of pregnancy with 20 tissue cysts of T. gondii (RRA strain), congenital infection was reduced from a control level of 50% to 12% in surviving offspring and foetal death from 50% to nil. The identification of protective antigens in the soluble fraction is well advanced.
AB - We have recently established that vertical disease transmission from the mother to the foetus, as in humans and sheep, only occurs in BALB/c mice infected with Toxoplasma gondii for first time during pregnancy. Thus a previous infection generally gives life long immunity, as ideally, would an effective vaccine. Of three major fractions harvested from RH strain tachyzoites (membrane, soluble and excretory/secretory) the soluble fraction was found the most effective in reducing mortality and cyst burdens in adult mice. Further studies indicated that the choice of adjuvant could enhance this effect. Entrapment within non-ionic surfactant vesicles (NISV), but not Freund’s Complete Adjuvant, significantly enhanced the protection afforded by soluble antigen to adult mice as well as greatly increasing the T cell specific proliferative response, IFN-γ production and antibody levels. NISV are safe, stable, and inexpensive and promote TH1 and CD8+ lymphocyte activation. In BALB/c dams vaccinated with NISV entrapped soluble antigen and infected on day 12 of pregnancy with 20 tissue cysts of T. gondii (RRA strain), congenital infection was reduced from a control level of 50% to 12% in surviving offspring and foetal death from 50% to nil. The identification of protective antigens in the soluble fraction is well advanced.
KW - toxoplasmosis
KW - antigens
UR - https://doi.org/10.1007/978-3-642-78559-7
U2 - 10.1007/978-3-642-78559-7_23
DO - 10.1007/978-3-642-78559-7_23
M3 - Chapter
SN - 9783642785610
VL - H78
T3 - Nato ASI Subseries H:
SP - 217
EP - 229
BT - Toxoplasmosis
A2 - Smith, Judith E.
PB - Springer-Verlag
CY - Berlin
ER -