Profiling primary protease specificity by peptide synthesis on a solid support

R H P Doeze, B A Maltman, C L Egan, R V Ulijn, S L Flitsch

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Reverse screening: A greatly simplified primary screening of protease specificity has been achieved by monitoring the fluorescence during the protease-catalyzed coupling of amino acids instead of peptide hydrolysis on a solid support (see picture, AA=amino acid). This approach paves the way for flexible, rapid, high-throughput identification and characterization of proteases without the need for expensively labeled peptide arrays.
LanguageEnglish
Pages3138-3141
Number of pages4
JournalAgewandte Chemie-International Edition
Volume43
Issue number24
DOIs
Publication statusPublished - 14 Jun 2004

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Peptides
Peptide Hydrolases
Amino acids
Screening
Amino Acids
Hydrolysis
Fluorescence
Throughput
Monitoring

Keywords

  • profiling
  • primary protease specificity
  • peptide synthesis
  • solid support
  • hydrolysis
  • substrate specificity
  • enzymes
  • fluorescent probe
  • high-throughput screening

Cite this

Doeze, R H P ; Maltman, B A ; Egan, C L ; Ulijn, R V ; Flitsch, S L . / Profiling primary protease specificity by peptide synthesis on a solid support. In: Agewandte Chemie-International Edition. 2004 ; Vol. 43, No. 24. pp. 3138-3141.
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Profiling primary protease specificity by peptide synthesis on a solid support. / Doeze, R H P ; Maltman, B A ; Egan, C L ; Ulijn, R V ; Flitsch, S L .

In: Agewandte Chemie-International Edition, Vol. 43, No. 24, 14.06.2004, p. 3138-3141.

Research output: Contribution to journalArticle

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AU - Doeze, R H P

AU - Maltman, B A

AU - Egan, C L

AU - Ulijn, R V

AU - Flitsch, S L

PY - 2004/6/14

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KW - profiling

KW - primary protease specificity

KW - peptide synthesis

KW - solid support

KW - hydrolysis

KW - substrate specificity

KW - enzymes

KW - fluorescent probe

KW - high-throughput screening

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DO - 10.1002/anie.200353367

M3 - Article

VL - 43

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JO - Angewandte Chemie International Edition

T2 - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

SN - 1433-7851

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