Profiling primary protease specificity by peptide synthesis on a solid support

R H P Doeze, B A Maltman, C L Egan, R V Ulijn, S L Flitsch

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Reverse screening: A greatly simplified primary screening of protease specificity has been achieved by monitoring the fluorescence during the protease-catalyzed coupling of amino acids instead of peptide hydrolysis on a solid support (see picture, AA=amino acid). This approach paves the way for flexible, rapid, high-throughput identification and characterization of proteases without the need for expensively labeled peptide arrays.
Original languageEnglish
Pages (from-to)3138-3141
Number of pages4
JournalAgewandte Chemie-International Edition
Volume43
Issue number24
DOIs
Publication statusPublished - 14 Jun 2004

Keywords

  • profiling
  • primary protease specificity
  • peptide synthesis
  • solid support
  • hydrolysis
  • substrate specificity
  • enzymes
  • fluorescent probe
  • high-throughput screening

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