Production of nanoscale vibration for stimulation of human mesenchymal stem cells

Habib Nikukar, Peter G Childs, Adam S G Curtis, Ian W Martin, Mathis O Riehle, Matthew J Dalby, Stuart Reid

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mechanical stimulation is becoming a common technique for manipulating cell behaviour in bioengineering with applications in tissue engineering and possibly regenerative therapy. Living organisms show biological responses in vivo and in vitro to various types of mechanical stimulation including vibration. The development of apparatus to produce vertical motions of nanoscale amplitude is detailed and their effect on mouse endothelial (Le2) and human mesenchymal stem cells (hMSCs) is investigated. Piezo ceramic actuators and aluminium reinforcement were utilised along with laser interferometry to ensure amplitude consistency at the nanometre level across a cell culture substrate. Peak force applied to the cells was estimated to be of nN magnitude at frequencies of 500 and 1000 Hz. Morphological changes in the cytoskeleton were found for both cell types along with increased MSC proliferation after 1 week of stimulation at 500 Hz. Changes in the nuclear size of MSCs after stimulation were also found.

Original languageEnglish
Pages (from-to)1478-88
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 2016

Fingerprint

Laser interferometry
Vibration
Stem cells
Aluminum
Mesenchymal Stromal Cells
Tissue engineering
Cell culture
Reinforcement
Actuators
Substrates
Interferometry
Bioengineering
Ceramics
Tissue Engineering
Cytoskeleton
Lasers
Cell Culture Techniques
Therapeutics

Keywords

  • Animals
  • Cell Culture Techniques
  • Cell Line
  • Cell Nucleus
  • Cell Proliferation
  • Humans
  • Mesenchymal Stromal Cells
  • Mice
  • Nanotechnology
  • Tissue Engineering
  • Vibration
  • Research Support, Non-U.S. Gov't
  • Journal Article

Cite this

Nikukar, H., Childs, P. G., Curtis, A. S. G., Martin, I. W., Riehle, M. O., Dalby, M. J., & Reid, S. (2016). Production of nanoscale vibration for stimulation of human mesenchymal stem cells. Journal of Biomedical Nanotechnology, 12(7), 1478-88. https://doi.org/10.1166/jbn.2016.2264
Nikukar, Habib ; Childs, Peter G ; Curtis, Adam S G ; Martin, Ian W ; Riehle, Mathis O ; Dalby, Matthew J ; Reid, Stuart. / Production of nanoscale vibration for stimulation of human mesenchymal stem cells. In: Journal of Biomedical Nanotechnology. 2016 ; Vol. 12, No. 7. pp. 1478-88.
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Nikukar, H, Childs, PG, Curtis, ASG, Martin, IW, Riehle, MO, Dalby, MJ & Reid, S 2016, 'Production of nanoscale vibration for stimulation of human mesenchymal stem cells', Journal of Biomedical Nanotechnology, vol. 12, no. 7, pp. 1478-88. https://doi.org/10.1166/jbn.2016.2264

Production of nanoscale vibration for stimulation of human mesenchymal stem cells. / Nikukar, Habib; Childs, Peter G; Curtis, Adam S G; Martin, Ian W; Riehle, Mathis O; Dalby, Matthew J; Reid, Stuart.

In: Journal of Biomedical Nanotechnology, Vol. 12, No. 7, 07.2016, p. 1478-88.

Research output: Contribution to journalArticle

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