Preservation methods of honey bee-collected pollen are not a source of bias in ITS2 metabarcoding

Andreia Quaresma; Robert Brodschneider; Kristina Gratzer; Alison Gray; Alexander Keller; Ole Kilpinen; José Rufino; Jozef van der Steen; Flemming Vejsnæs; M. Alice Pinto

doi:10.1007/s10661-021-09563-4

Preservation methods of honey bee-collected pollen are not a source of bias in ITS2 metabarcoding

Andreia Quaresma, Robert Brodschneider, Kristina Gratzer, Alison Gray, Alexander Keller, Ole Kilpinen, José Rufino, Jozef van der Steen, Flemming Vejsnæs, M. Alice Pinto

Mathematics And Statistics

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Abstract

Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen has been preserved at - 20 °C (FRZ), this is not the best method for citizen scientists. Herein, we compared this method with ethanol (EtOH), silica gel (SG) and room temperature (RT) for preservation of pollen collected from hives in Austria and Denmark. After ~ 4 months of storage, DNAs were extracted with a food kit, and their quality and concentration measured. Most DNA extracts exhibited 260/280 absorbance ratios close to the optimal 1.8, with RT samples from Austria performing slightly worse than FRZ and SG samples (P

Original language	English
Article number	785
Number of pages	20
Journal	Environmental Monitoring and Assessment
Volume	193
Issue number	12
DOIs	https://doi.org/10.1007/s10661-021-09563-4
Publication status	Published - 9 Nov 2021

Keywords

DNA Barcoding, Taxonomic
Citizen science
Pollen DNA barcoding
Animals
Honey
Bees
DNA metabarcoding
Environmental Monitoring
Silica gel preservation
Preservation bias
Pollen
Bias

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10.1007/s10661-021-09563-4

Quaresma-etal-EMA-2021-Preservation-methods-of-honey-bee-collected-pollen-are-not-a-source-of-bias-in-ITS2-metabarcodingFinal published version, 3.19 MBLicence: CC BY 4.0

Citizen Science Investigation for Pesticides in Apicultural Products (INSIGNIA)
Gray, A. (Principal Investigator)
European Commission - Horizon Europe + H2020
1/11/18 → 30/04/21
Project: Research

Cite this

@article{69c60b53e20649fdafc40906aec76a5c,

title = "Preservation methods of honey bee-collected pollen are not a source of bias in ITS2 metabarcoding",

abstract = "Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen has been preserved at - 20 °C (FRZ), this is not the best method for citizen scientists. Herein, we compared this method with ethanol (EtOH), silica gel (SG) and room temperature (RT) for preservation of pollen collected from hives in Austria and Denmark. After ~ 4 months of storage, DNAs were extracted with a food kit, and their quality and concentration measured. Most DNA extracts exhibited 260/280 absorbance ratios close to the optimal 1.8, with RT samples from Austria performing slightly worse than FRZ and SG samples (P ",

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author = "Andreia Quaresma and Robert Brodschneider and Kristina Gratzer and Alison Gray and Alexander Keller and Ole Kilpinen and Jos{\'e} Rufino and {van der Steen}, Jozef and Flemming Vejsn{\ae}s and Pinto, {M. Alice}",

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AU - Quaresma, Andreia

AU - Brodschneider, Robert

AU - Gratzer, Kristina

AU - Gray, Alison

AU - Keller, Alexander

AU - Kilpinen, Ole

AU - Rufino, José

AU - van der Steen, Jozef

AU - Vejsnæs, Flemming

AU - Pinto, M. Alice

PY - 2021/11/9

Y1 - 2021/11/9

N2 - Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen has been preserved at - 20 °C (FRZ), this is not the best method for citizen scientists. Herein, we compared this method with ethanol (EtOH), silica gel (SG) and room temperature (RT) for preservation of pollen collected from hives in Austria and Denmark. After ~ 4 months of storage, DNAs were extracted with a food kit, and their quality and concentration measured. Most DNA extracts exhibited 260/280 absorbance ratios close to the optimal 1.8, with RT samples from Austria performing slightly worse than FRZ and SG samples (P

AB - Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen has been preserved at - 20 °C (FRZ), this is not the best method for citizen scientists. Herein, we compared this method with ethanol (EtOH), silica gel (SG) and room temperature (RT) for preservation of pollen collected from hives in Austria and Denmark. After ~ 4 months of storage, DNAs were extracted with a food kit, and their quality and concentration measured. Most DNA extracts exhibited 260/280 absorbance ratios close to the optimal 1.8, with RT samples from Austria performing slightly worse than FRZ and SG samples (P

KW - DNA Barcoding, Taxonomic

KW - Citizen science

KW - Pollen DNA barcoding

KW - Animals

KW - Honey

KW - Bees

KW - DNA metabarcoding

KW - Environmental Monitoring

KW - Silica gel preservation

KW - Preservation bias

KW - Pollen

KW - Bias

U2 - 10.1007/s10661-021-09563-4

DO - 10.1007/s10661-021-09563-4

M3 - Article

C2 - 34755261

SN - 0167-6369

VL - 193

JO - Environmental Monitoring and Assessment

JF - Environmental Monitoring and Assessment

IS - 12

M1 - 785

ER -

Preservation methods of honey bee-collected pollen are not a source of bias in ITS2 metabarcoding

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Keywords

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Projects

Citizen Science Investigation for Pesticides in Apicultural Products (INSIGNIA)

Cite this