Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

Johanna M C Jefferies, Calum H G Johnston, Lea-Ann S Kirkham, Graeme J M Cowan, Kirsty S Ross, Andrew Smith, Stuart C Clarke, Angela B Brueggemann, Robert C George, Bruno Pichon, Gerd Pluschke, Valentin Pfluger, Tim J Mitchell

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.
Original languageEnglish
Pages (from-to)936-944
Number of pages9
JournalJournal of Infectious Diseases
Volume196
Issue number6
DOIs
Publication statusPublished - 15 Sep 2007

Fingerprint

Streptococcus pneumoniae
Disease Outbreaks
Alleles
Virulence Factors
Sequence Analysis
Proteins
Clone Cells
Serogroup
Streptococcus pneumoniae plY protein
Genes

Keywords

  • alleles
  • bacterial proteins
  • cluster analysis
  • DNA, bacterial
  • disease outbreaks
  • genotype
  • hemolysis
  • humans
  • molecular sequence data
  • pneumococcal infections
  • polymorphism, genetic
  • sequence analysis, DNA
  • sequence homology
  • serotyping
  • streptococcus pneumoniae
  • streptolysins
  • virulence factors

Cite this

Jefferies, J. M. C., Johnston, C. H. G., Kirkham, L-A. S., Cowan, G. J. M., Ross, K. S., Smith, A., ... Mitchell, T. J. (2007). Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks. Journal of Infectious Diseases, 196(6), 936-944. https://doi.org/10.1086/520091
Jefferies, Johanna M C ; Johnston, Calum H G ; Kirkham, Lea-Ann S ; Cowan, Graeme J M ; Ross, Kirsty S ; Smith, Andrew ; Clarke, Stuart C ; Brueggemann, Angela B ; George, Robert C ; Pichon, Bruno ; Pluschke, Gerd ; Pfluger, Valentin ; Mitchell, Tim J. / Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks. In: Journal of Infectious Diseases. 2007 ; Vol. 196, No. 6. pp. 936-944.
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abstract = "Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.",
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Jefferies, JMC, Johnston, CHG, Kirkham, L-AS, Cowan, GJM, Ross, KS, Smith, A, Clarke, SC, Brueggemann, AB, George, RC, Pichon, B, Pluschke, G, Pfluger, V & Mitchell, TJ 2007, 'Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks', Journal of Infectious Diseases, vol. 196, no. 6, pp. 936-944. https://doi.org/10.1086/520091

Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks. / Jefferies, Johanna M C; Johnston, Calum H G; Kirkham, Lea-Ann S; Cowan, Graeme J M; Ross, Kirsty S; Smith, Andrew; Clarke, Stuart C; Brueggemann, Angela B; George, Robert C; Pichon, Bruno; Pluschke, Gerd; Pfluger, Valentin; Mitchell, Tim J.

In: Journal of Infectious Diseases, Vol. 196, No. 6, 15.09.2007, p. 936-944.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

AU - Jefferies, Johanna M C

AU - Johnston, Calum H G

AU - Kirkham, Lea-Ann S

AU - Cowan, Graeme J M

AU - Ross, Kirsty S

AU - Smith, Andrew

AU - Clarke, Stuart C

AU - Brueggemann, Angela B

AU - George, Robert C

AU - Pichon, Bruno

AU - Pluschke, Gerd

AU - Pfluger, Valentin

AU - Mitchell, Tim J

PY - 2007/9/15

Y1 - 2007/9/15

N2 - Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

AB - Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

KW - alleles

KW - bacterial proteins

KW - cluster analysis

KW - DNA, bacterial

KW - disease outbreaks

KW - genotype

KW - hemolysis

KW - humans

KW - molecular sequence data

KW - pneumococcal infections

KW - polymorphism, genetic

KW - sequence analysis, DNA

KW - sequence homology

KW - serotyping

KW - streptococcus pneumoniae

KW - streptolysins

KW - virulence factors

U2 - 10.1086/520091

DO - 10.1086/520091

M3 - Article

VL - 196

SP - 936

EP - 944

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 6

ER -