Precision printing of living biological cells

Marcus Ardron, Mihails Bogomolnijs, Alan Faulkner-Jones, Godfrey L Smith, Victor Zamora Rodriguez, Wenmiao Shu

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

Living tissues can be constructed in vitro by 3D bioprinting of cells held in hydrogel solutions which are then cross-linked to form 3D geometries analogous to in vivo tissues. Signalling from cell- To-cell and microenvironment- To-cell interaction both effect cell behaviour and life cycle. Artificial cell containing constructs must mimic a sufficient level of the complexity of in vivo tissues to be useful in vitro models for drug testing and fundamental cell biology research. Further demands include maintaining cellular viability and functions during tissue construction while also minimising printing time, cells, cell culture media and reagents required. Our work involves interdisciplinary collaboration in the fields of bioengineering, stem cell biology, precision engineering and process control. Together we are building a high precision living cell printing system capable of rapidly constructing complex tissues in 3D. A number of highly sensitive cell types have been successfully printed including human pluripotent stem cell derived cardiomyocytes and hepatocyte-like cells. Cell printing, 2D & 3D constructs, plus a positioning mechanism were all complemented by diagnostics to give the building blocks needed for the manufacture of artificial biological screening models.

LanguageEnglish
Title of host publicationProceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK
Place of PublicationBedford, UK
Publication statusPublished - 3 Jun 2016
Externally publishedYes
Event16th International Conference of the European Society for Precision Engineering and Nanotechnology, EUSPEN 2016 - Nottingham, United Kingdom
Duration: 30 May 20163 Jun 2016

Conference

Conference16th International Conference of the European Society for Precision Engineering and Nanotechnology, EUSPEN 2016
CountryUnited Kingdom
CityNottingham
Period30/05/163/06/16

Fingerprint

printing
Printing
Tissue
Cytology
cells
Stem cells
Precision engineering
stem cells
Hydrogel
Cell culture
Hydrogels
Process control
Culture Media
Life cycle
Screening
Cells
bioengineering
culture media
Geometry
Testing

Keywords

  • bioprinting
  • cardiomyocytes
  • screening
  • stem cells
  • toxicology

Cite this

Ardron, M., Bogomolnijs, M., Faulkner-Jones, A., Smith, G. L., Rodriguez, V. Z., & Shu, W. (2016). Precision printing of living biological cells. In Proceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK Bedford, UK.
Ardron, Marcus ; Bogomolnijs, Mihails ; Faulkner-Jones, Alan ; Smith, Godfrey L ; Rodriguez, Victor Zamora ; Shu, Wenmiao. / Precision printing of living biological cells. Proceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK. Bedford, UK, 2016.
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Ardron, M, Bogomolnijs, M, Faulkner-Jones, A, Smith, GL, Rodriguez, VZ & Shu, W 2016, Precision printing of living biological cells. in Proceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK. Bedford, UK, 16th International Conference of the European Society for Precision Engineering and Nanotechnology, EUSPEN 2016, Nottingham, United Kingdom, 30/05/16.

Precision printing of living biological cells. / Ardron, Marcus; Bogomolnijs, Mihails; Faulkner-Jones, Alan; Smith, Godfrey L; Rodriguez, Victor Zamora; Shu, Wenmiao.

Proceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK. Bedford, UK, 2016.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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AB - Living tissues can be constructed in vitro by 3D bioprinting of cells held in hydrogel solutions which are then cross-linked to form 3D geometries analogous to in vivo tissues. Signalling from cell- To-cell and microenvironment- To-cell interaction both effect cell behaviour and life cycle. Artificial cell containing constructs must mimic a sufficient level of the complexity of in vivo tissues to be useful in vitro models for drug testing and fundamental cell biology research. Further demands include maintaining cellular viability and functions during tissue construction while also minimising printing time, cells, cell culture media and reagents required. Our work involves interdisciplinary collaboration in the fields of bioengineering, stem cell biology, precision engineering and process control. Together we are building a high precision living cell printing system capable of rapidly constructing complex tissues in 3D. A number of highly sensitive cell types have been successfully printed including human pluripotent stem cell derived cardiomyocytes and hepatocyte-like cells. Cell printing, 2D & 3D constructs, plus a positioning mechanism were all complemented by diagnostics to give the building blocks needed for the manufacture of artificial biological screening models.

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Ardron M, Bogomolnijs M, Faulkner-Jones A, Smith GL, Rodriguez VZ, Shu W. Precision printing of living biological cells. In Proceedings of the 16th International Conference of the European Society for Precision Engineering and Nanotechnology May 30th-3rd June 2016, Nottingham, UK. Bedford, UK. 2016