Abstract
| Language | English |
|---|---|
| Pages | 263-272 |
| Number of pages | 10 |
| Journal | Journal of Controlled Release |
| Volume | 106 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Sep 2005 |
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Keywords
- poly(ε-caprolactone)
- microporous
- matrix
- progesterone
- MCF-7 cells
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Precipitation casting of drug-loaded microporous PCL matrices : incorporation of progesterone by co-dissolution. / Chang, H.-I.; Williamson, M.R.; Perrie, Yvonne; Coombes, Allan G.A.
In: Journal of Controlled Release, Vol. 106, No. 3, 01.09.2005, p. 263-272.Research output: Contribution to journal › Article
TY - JOUR
T1 - Precipitation casting of drug-loaded microporous PCL matrices
T2 - Journal of Controlled Release
AU - Chang, H.-I.
AU - Williamson, M.R.
AU - Perrie, Yvonne
AU - Coombes, Allan G.A.
PY - 2005/9/1
Y1 - 2005/9/1
N2 - Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with progesterone by precipitation casting using co-solutions of PCL and progesterone in acetone. Progesterone loadings up to 32% w/w were readily achieved by increasing the drug content of the starting PCL solution. The kinetics of steroid release in PBS at 37 °C over 10 days could be described effectively by a diffusional release model although the Korsmeyer–Peppas model indicated the involvement of multiple release phenomena. The diffusion rate constant (D) increased from 8 to 24 μg/mg matrix/day0.5 as the drug loading increased from 3.6 to 12.4% w/w. A total cumulative release of 75%–95% indicates the high efficiency of steroid delivery. Increasing the matrix density from 0.22 to 0.39 g/cm3, by increasing the starting PCL solution concentration, was less effective in changing drug release kinetics. Retention of anti-proliferative activity of released steroid was confirmed using cultures of breast cancer epithelial (MCF-7) cells. Progesterone released from PCL matrices into PBS at 37 °C over 14 days retarded the growth of MCF-7 cells by a factor of at least 3.5 compared with progesterone-free controls. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of bioactive molecules such as anti-proliferative agents from implanted, inserted or topical devices.
AB - Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with progesterone by precipitation casting using co-solutions of PCL and progesterone in acetone. Progesterone loadings up to 32% w/w were readily achieved by increasing the drug content of the starting PCL solution. The kinetics of steroid release in PBS at 37 °C over 10 days could be described effectively by a diffusional release model although the Korsmeyer–Peppas model indicated the involvement of multiple release phenomena. The diffusion rate constant (D) increased from 8 to 24 μg/mg matrix/day0.5 as the drug loading increased from 3.6 to 12.4% w/w. A total cumulative release of 75%–95% indicates the high efficiency of steroid delivery. Increasing the matrix density from 0.22 to 0.39 g/cm3, by increasing the starting PCL solution concentration, was less effective in changing drug release kinetics. Retention of anti-proliferative activity of released steroid was confirmed using cultures of breast cancer epithelial (MCF-7) cells. Progesterone released from PCL matrices into PBS at 37 °C over 14 days retarded the growth of MCF-7 cells by a factor of at least 3.5 compared with progesterone-free controls. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of bioactive molecules such as anti-proliferative agents from implanted, inserted or topical devices.
KW - poly(ε-caprolactone)
KW - microporous
KW - matrix
KW - progesterone
KW - MCF-7 cells
UR - http://www.sciencedirect.com/science/article/pii/S0168365905002294
U2 - 10.1016/j.jconrel.2005.05.013
DO - 10.1016/j.jconrel.2005.05.013
M3 - Article
VL - 106
SP - 263
EP - 272
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 3
ER -