Practical implementation of adaptive optics in multiphoton microscopy

P.N. Marsh, D. Burns, J.M. Girkin

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.
LanguageEnglish
Pages1123-1130
Number of pages7
JournalOptics Express
Volume11
Issue number10
Publication statusPublished - 2003

Fingerprint

adaptive optics
aberration
microscopy
deformable mirrors
point spread functions
submerging
modules
oils
microscopes
laser beams
refractivity
fluorescence
scanning
photons
water

Keywords

  • optics
  • photonics
  • lasers
  • quantum electronics

Cite this

Marsh, P. N., Burns, D., & Girkin, J. M. (2003). Practical implementation of adaptive optics in multiphoton microscopy. Optics Express, 11(10), 1123-1130.
Marsh, P.N. ; Burns, D. ; Girkin, J.M. / Practical implementation of adaptive optics in multiphoton microscopy. In: Optics Express. 2003 ; Vol. 11, No. 10. pp. 1123-1130.
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Marsh, PN, Burns, D & Girkin, JM 2003, 'Practical implementation of adaptive optics in multiphoton microscopy' Optics Express, vol. 11, no. 10, pp. 1123-1130.

Practical implementation of adaptive optics in multiphoton microscopy. / Marsh, P.N.; Burns, D.; Girkin, J.M.

In: Optics Express, Vol. 11, No. 10, 2003, p. 1123-1130.

Research output: Contribution to journalArticle

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Marsh PN, Burns D, Girkin JM. Practical implementation of adaptive optics in multiphoton microscopy. Optics Express. 2003;11(10):1123-1130.