Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells

Hajime Nakatani, Takehiko Yasueda, Kenzi Oshima, Tetsuya Okajima, Daita Nadano, David J Flint, Tsukasa Matsuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Milk fat globules (MFGs) secreted by lactating mammary gland are unique lipid surrounded by a phospholipid bi-layer. We report here post-weaning changes in milk fat globule EGF factor VIII (MFG-E8) and annexin V-accessible phosphatidylserine on the surface of MFGs. The MFG content in milk markedly decreased to about half within 2 days after forced weaning, despite a slight increase in milk protein content. Immunofluorescence-staining of MFGs using anti-MFG-E8 and annexin V indicated that MFG-E8 was present on some, but not all, MFGs before weaning, whereas most of MFGs were MFG-E8-positive and annexin V-negative after weaning. Free MFG-E8 with binding activity to phosphatidylserine was present abundantly in the post-weaning milk, and indeed exhibited binding to MFGs in pre-weaning milk. MFGs were taken up by HC11 mouse mammary epithelial cells in vitro, and those from post-weaning milk were remarkable for such cellular uptake. Moreover, the uptake of MFGs by the cells was inhibited by an anti-MFG-E8 antibody. Taken together, these findings suggest that MFG-E8 plays a critical role in regulation of MFG dynamics after weaning or during the suckling interval through the control of MFG-epithelial cell interaction in lactating mammary glands.
LanguageEnglish
Pages31-41
Number of pages11
JournalJournal of Biochemistry
Volume153
Issue number1
Early online date3 Oct 2012
DOIs
Publication statusPublished - 31 Jan 2013

Fingerprint

Factor VIII
Weaning
Epidermal Growth Factor
Milk
Breast
Epithelial Cells
Fats
Annexin A5
milk fat globule
Phosphatidylserines
Human Mammary Glands
Milk Proteins

Keywords

  • mammary gland
  • lactation
  • involution
  • milk fat globule membrane
  • fat metabolism

Cite this

Nakatani, Hajime ; Yasueda, Takehiko ; Oshima, Kenzi ; Okajima, Tetsuya ; Nadano, Daita ; Flint, David J ; Matsuda, Tsukasa. / Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells. In: Journal of Biochemistry. 2013 ; Vol. 153, No. 1. pp. 31-41.
@article{d42608ad46f1413badfb8cafdba3249d,
title = "Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells",
abstract = "Milk fat globules (MFGs) secreted by lactating mammary gland are unique lipid surrounded by a phospholipid bi-layer. We report here post-weaning changes in milk fat globule EGF factor VIII (MFG-E8) and annexin V-accessible phosphatidylserine on the surface of MFGs. The MFG content in milk markedly decreased to about half within 2 days after forced weaning, despite a slight increase in milk protein content. Immunofluorescence-staining of MFGs using anti-MFG-E8 and annexin V indicated that MFG-E8 was present on some, but not all, MFGs before weaning, whereas most of MFGs were MFG-E8-positive and annexin V-negative after weaning. Free MFG-E8 with binding activity to phosphatidylserine was present abundantly in the post-weaning milk, and indeed exhibited binding to MFGs in pre-weaning milk. MFGs were taken up by HC11 mouse mammary epithelial cells in vitro, and those from post-weaning milk were remarkable for such cellular uptake. Moreover, the uptake of MFGs by the cells was inhibited by an anti-MFG-E8 antibody. Taken together, these findings suggest that MFG-E8 plays a critical role in regulation of MFG dynamics after weaning or during the suckling interval through the control of MFG-epithelial cell interaction in lactating mammary glands.",
keywords = "mammary gland, lactation, involution, milk fat globule membrane, fat metabolism",
author = "Hajime Nakatani and Takehiko Yasueda and Kenzi Oshima and Tetsuya Okajima and Daita Nadano and Flint, {David J} and Tsukasa Matsuda",
year = "2013",
month = "1",
day = "31",
doi = "10.1093/jb/mvs116",
language = "English",
volume = "153",
pages = "31--41",
journal = "Journal of Biochemistry",
issn = "0021-924X",
number = "1",

}

Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells. / Nakatani, Hajime; Yasueda, Takehiko; Oshima, Kenzi; Okajima, Tetsuya; Nadano, Daita; Flint, David J; Matsuda, Tsukasa.

In: Journal of Biochemistry, Vol. 153, No. 1, 31.01.2013, p. 31-41.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Post-weaning increases in the milk-fat globule EGF-factor VIII on fat globules in mouse milk and in the uptake of the fat globules by HC11 mammary epithelial cells

AU - Nakatani, Hajime

AU - Yasueda, Takehiko

AU - Oshima, Kenzi

AU - Okajima, Tetsuya

AU - Nadano, Daita

AU - Flint, David J

AU - Matsuda, Tsukasa

PY - 2013/1/31

Y1 - 2013/1/31

N2 - Milk fat globules (MFGs) secreted by lactating mammary gland are unique lipid surrounded by a phospholipid bi-layer. We report here post-weaning changes in milk fat globule EGF factor VIII (MFG-E8) and annexin V-accessible phosphatidylserine on the surface of MFGs. The MFG content in milk markedly decreased to about half within 2 days after forced weaning, despite a slight increase in milk protein content. Immunofluorescence-staining of MFGs using anti-MFG-E8 and annexin V indicated that MFG-E8 was present on some, but not all, MFGs before weaning, whereas most of MFGs were MFG-E8-positive and annexin V-negative after weaning. Free MFG-E8 with binding activity to phosphatidylserine was present abundantly in the post-weaning milk, and indeed exhibited binding to MFGs in pre-weaning milk. MFGs were taken up by HC11 mouse mammary epithelial cells in vitro, and those from post-weaning milk were remarkable for such cellular uptake. Moreover, the uptake of MFGs by the cells was inhibited by an anti-MFG-E8 antibody. Taken together, these findings suggest that MFG-E8 plays a critical role in regulation of MFG dynamics after weaning or during the suckling interval through the control of MFG-epithelial cell interaction in lactating mammary glands.

AB - Milk fat globules (MFGs) secreted by lactating mammary gland are unique lipid surrounded by a phospholipid bi-layer. We report here post-weaning changes in milk fat globule EGF factor VIII (MFG-E8) and annexin V-accessible phosphatidylserine on the surface of MFGs. The MFG content in milk markedly decreased to about half within 2 days after forced weaning, despite a slight increase in milk protein content. Immunofluorescence-staining of MFGs using anti-MFG-E8 and annexin V indicated that MFG-E8 was present on some, but not all, MFGs before weaning, whereas most of MFGs were MFG-E8-positive and annexin V-negative after weaning. Free MFG-E8 with binding activity to phosphatidylserine was present abundantly in the post-weaning milk, and indeed exhibited binding to MFGs in pre-weaning milk. MFGs were taken up by HC11 mouse mammary epithelial cells in vitro, and those from post-weaning milk were remarkable for such cellular uptake. Moreover, the uptake of MFGs by the cells was inhibited by an anti-MFG-E8 antibody. Taken together, these findings suggest that MFG-E8 plays a critical role in regulation of MFG dynamics after weaning or during the suckling interval through the control of MFG-epithelial cell interaction in lactating mammary glands.

KW - mammary gland

KW - lactation

KW - involution

KW - milk fat globule membrane

KW - fat metabolism

UR - http://www.scopus.com/inward/record.url?scp=84871629394&partnerID=8YFLogxK

U2 - 10.1093/jb/mvs116

DO - 10.1093/jb/mvs116

M3 - Article

VL - 153

SP - 31

EP - 41

JO - Journal of Biochemistry

T2 - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -