Possible developments in neurotoxicity testing in vitro

Research output: Contribution to journalLiterature review

6 Citations (Scopus)

Abstract

1. Many different chemical compounds produce a range of neurotoxic effects. They do not share a common mechanism and there is no single universally applicable test for neurotoxicity. 2. Testing for neurotoxicity in vivo is not always completely reliable, and many neurotoxic effects are still discovered following accidental exposure to humans. 3. Given the complexity of neurotoxins and their effects in vivo and the difficulties of elucidation of such effects in vivo, there is a place for the use of simple in vitro model systems in neutrotoxicity testing. 4. Various preparations of invertebrate neurones have been used in toxicological studies because of the similarities in basic neuronal properties between invertebrates and higher species. Mammalian nerve cells can be grown in culture and they are suitable for biochemical and electrophysiological experiments. Such neuronal cultures could also be adapted for toxicological studies. Cultures of continuous cell lines would probably be more convenient than primary cultures. 5. Future developments should concentrate on the establishment of a battery of human hybrid cell lines and of a range of biochemical assays. After validation by experiments with a series of known neurotoxins, such cultures would be suitable for neurotoxicological tests.
Original languageEnglish
Pages (from-to)625-632
Number of pages8
JournalXenobiotica
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 1988

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Neurotoxins
Invertebrates
Toxicology
Neurons
Cells
Cell Line
Chemical compounds
Hybrid Cells
Testing
Cell culture
Assays
Experiments
In Vitro Techniques

Keywords

  • animal testing alternatives
  • animals
  • cultured cells, Cultured
  • chickens
  • preclinical drug evaluation
  • muscles
  • neurons
  • neurotoxins
  • snake venoms

Cite this

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title = "Possible developments in neurotoxicity testing in vitro",
abstract = "1. Many different chemical compounds produce a range of neurotoxic effects. They do not share a common mechanism and there is no single universally applicable test for neurotoxicity. 2. Testing for neurotoxicity in vivo is not always completely reliable, and many neurotoxic effects are still discovered following accidental exposure to humans. 3. Given the complexity of neurotoxins and their effects in vivo and the difficulties of elucidation of such effects in vivo, there is a place for the use of simple in vitro model systems in neutrotoxicity testing. 4. Various preparations of invertebrate neurones have been used in toxicological studies because of the similarities in basic neuronal properties between invertebrates and higher species. Mammalian nerve cells can be grown in culture and they are suitable for biochemical and electrophysiological experiments. Such neuronal cultures could also be adapted for toxicological studies. Cultures of continuous cell lines would probably be more convenient than primary cultures. 5. Future developments should concentrate on the establishment of a battery of human hybrid cell lines and of a range of biochemical assays. After validation by experiments with a series of known neurotoxins, such cultures would be suitable for neurotoxicological tests.",
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Possible developments in neurotoxicity testing in vitro. / Harvey, A L.

In: Xenobiotica, Vol. 18, No. 6, 06.1988, p. 625-632.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - Possible developments in neurotoxicity testing in vitro

AU - Harvey, A L

PY - 1988/6

Y1 - 1988/6

N2 - 1. Many different chemical compounds produce a range of neurotoxic effects. They do not share a common mechanism and there is no single universally applicable test for neurotoxicity. 2. Testing for neurotoxicity in vivo is not always completely reliable, and many neurotoxic effects are still discovered following accidental exposure to humans. 3. Given the complexity of neurotoxins and their effects in vivo and the difficulties of elucidation of such effects in vivo, there is a place for the use of simple in vitro model systems in neutrotoxicity testing. 4. Various preparations of invertebrate neurones have been used in toxicological studies because of the similarities in basic neuronal properties between invertebrates and higher species. Mammalian nerve cells can be grown in culture and they are suitable for biochemical and electrophysiological experiments. Such neuronal cultures could also be adapted for toxicological studies. Cultures of continuous cell lines would probably be more convenient than primary cultures. 5. Future developments should concentrate on the establishment of a battery of human hybrid cell lines and of a range of biochemical assays. After validation by experiments with a series of known neurotoxins, such cultures would be suitable for neurotoxicological tests.

AB - 1. Many different chemical compounds produce a range of neurotoxic effects. They do not share a common mechanism and there is no single universally applicable test for neurotoxicity. 2. Testing for neurotoxicity in vivo is not always completely reliable, and many neurotoxic effects are still discovered following accidental exposure to humans. 3. Given the complexity of neurotoxins and their effects in vivo and the difficulties of elucidation of such effects in vivo, there is a place for the use of simple in vitro model systems in neutrotoxicity testing. 4. Various preparations of invertebrate neurones have been used in toxicological studies because of the similarities in basic neuronal properties between invertebrates and higher species. Mammalian nerve cells can be grown in culture and they are suitable for biochemical and electrophysiological experiments. Such neuronal cultures could also be adapted for toxicological studies. Cultures of continuous cell lines would probably be more convenient than primary cultures. 5. Future developments should concentrate on the establishment of a battery of human hybrid cell lines and of a range of biochemical assays. After validation by experiments with a series of known neurotoxins, such cultures would be suitable for neurotoxicological tests.

KW - animal testing alternatives

KW - animals

KW - cultured cells, Cultured

KW - chickens

KW - preclinical drug evaluation

KW - muscles

KW - neurons

KW - neurotoxins

KW - snake venoms

U2 - 10.3109/00498258809041700

DO - 10.3109/00498258809041700

M3 - Literature review

VL - 18

SP - 625

EP - 632

JO - Xenobiotica

JF - Xenobiotica

SN - 0049-8254

IS - 6

ER -