Plasticity of streptomyces coelicolor membrane composition under different growth conditions and during development

Mario Sandoval-Calderón, Don D. Nguyen, Clifford A. Kapono, Paul Herron, Pieter C. Dorrestein, Christian Sohlenkamp

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Abstract

Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor.

Original languageEnglish
Article number1465
Number of pages13
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - 22 Dec 2015

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Streptomyces coelicolor
Lipids
Membranes
Growth
Phosphorus
Secondary Metabolism
Actinobacteria
Streptomyces
Membrane Lipids
Life Cycle Stages
Ecosystem
Soil
Phosphates
Cell Membrane
Genes
ornithine containing aminolipid

Keywords

  • streptomyces coelicolor
  • lipid composition
  • phosphatidylethanolamine
  • bacterial development
  • phospholipids
  • ornithine lipids
  • phosphorus limitation
  • membrane adaptation

Cite this

Sandoval-Calderón, Mario ; Nguyen, Don D. ; Kapono, Clifford A. ; Herron, Paul ; Dorrestein, Pieter C. ; Sohlenkamp, Christian. / Plasticity of streptomyces coelicolor membrane composition under different growth conditions and during development. In: Frontiers in Microbiology. 2015 ; Vol. 6.
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Plasticity of streptomyces coelicolor membrane composition under different growth conditions and during development. / Sandoval-Calderón, Mario; Nguyen, Don D.; Kapono, Clifford A.; Herron, Paul; Dorrestein, Pieter C.; Sohlenkamp, Christian.

In: Frontiers in Microbiology, Vol. 6, 1465, 22.12.2015.

Research output: Contribution to journalArticle

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