Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth

Roberto De La Rica, Laura Rodriguez-Lorenzo, Ramon Alvarez-Puebla, Luis Liz-Marzan, Molly Stevens

Research output: Contribution to journalLetter

237 Citations (Scopus)

Abstract

Lowering the limit of detection is key to the design of sensors needed for food safety regulations1, 2, environmental policies3, 4, 5 and the diagnosis of severe diseases6, 7, 8, 9, 10. However, because conventional transducers generate a signal that is directly proportional to the concentration of the target molecule, ultralow concentrations of the molecule result in variations in the physical properties of the sensor that are tiny, and therefore difficult to detect with confidence. Here we present a signal-generation mechanism that redefines the limit of detection of nanoparticle sensors by inducing a signal that is larger when the target molecule is less concentrated. The key step to achieve this inverse sensitivity is to use an enzyme that controls the rate of nucleation of silver nanocrystals on plasmonic transducers. We demonstrate the outstanding sensitivity and robustness of this approach by detecting the cancer biomarker prostate-specific antigen down to 10−18 g ml−1 (4 × 10−20 M) in whole serum.
Original languageEnglish
Pages (from-to)604–607
Number of pages4
JournalNature Materials
Volume11
Early online date27 May 2012
DOIs
Publication statusPublished - 2012

Fingerprint

Nanosensors
Crystallization
Crystal growth
enzymes
crystal growth
Enzymes
Molecules
Transducers
sensitivity
sensors
Sensors
transducers
Food safety
molecules
biomarkers
antigens
Prostate-Specific Antigen
Antigens
Tumor Biomarkers
Silver

Keywords

  • plasmonic nanosensors
  • inverse sensitivity
  • enzyme-guided
  • crystal growth

Cite this

De La Rica, R., Rodriguez-Lorenzo, L., Alvarez-Puebla, R., Liz-Marzan, L., & Stevens, M. (2012). Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. Nature Materials, 11, 604–607. https://doi.org/10.1038/nmat3337
De La Rica, Roberto ; Rodriguez-Lorenzo, Laura ; Alvarez-Puebla, Ramon ; Liz-Marzan, Luis ; Stevens, Molly. / Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. In: Nature Materials. 2012 ; Vol. 11. pp. 604–607.
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De La Rica, R, Rodriguez-Lorenzo, L, Alvarez-Puebla, R, Liz-Marzan, L & Stevens, M 2012, 'Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth', Nature Materials, vol. 11, pp. 604–607. https://doi.org/10.1038/nmat3337

Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. / De La Rica, Roberto; Rodriguez-Lorenzo, Laura; Alvarez-Puebla, Ramon; Liz-Marzan, Luis; Stevens, Molly.

In: Nature Materials, Vol. 11, 2012, p. 604–607.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth

AU - De La Rica, Roberto

AU - Rodriguez-Lorenzo, Laura

AU - Alvarez-Puebla, Ramon

AU - Liz-Marzan, Luis

AU - Stevens, Molly

PY - 2012

Y1 - 2012

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AB - Lowering the limit of detection is key to the design of sensors needed for food safety regulations1, 2, environmental policies3, 4, 5 and the diagnosis of severe diseases6, 7, 8, 9, 10. However, because conventional transducers generate a signal that is directly proportional to the concentration of the target molecule, ultralow concentrations of the molecule result in variations in the physical properties of the sensor that are tiny, and therefore difficult to detect with confidence. Here we present a signal-generation mechanism that redefines the limit of detection of nanoparticle sensors by inducing a signal that is larger when the target molecule is less concentrated. The key step to achieve this inverse sensitivity is to use an enzyme that controls the rate of nucleation of silver nanocrystals on plasmonic transducers. We demonstrate the outstanding sensitivity and robustness of this approach by detecting the cancer biomarker prostate-specific antigen down to 10−18 g ml−1 (4 × 10−20 M) in whole serum.

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KW - inverse sensitivity

KW - enzyme-guided

KW - crystal growth

U2 - 10.1038/nmat3337

DO - 10.1038/nmat3337

M3 - Letter

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ER -

De La Rica R, Rodriguez-Lorenzo L, Alvarez-Puebla R, Liz-Marzan L, Stevens M. Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. Nature Materials. 2012;11:604–607. https://doi.org/10.1038/nmat3337