Plasma free fatty acids metabolic profile with LC-MS and appetite-related hormones in South Asian and white European men in relation to adiposity, physical activity and cardiorespiratory fitness: a cross-sectional study

Simone Benedetti, Naser F. Al-Tannak, Mansour Alzharani, Hannah J. Moir, David J. Stensel, Alice E. Thackray, Declan P. Naughton, Mehmet T. Dorak, Owen Spendif, Natasha Hill, David Watson, Judith Allgrove

Research output: Contribution to journalArticle

Abstract

South Asians have a greater cardiovascular disease (CVD) and type 2 diabetes (T2D) risk than white Europeans, but the mechanisms are poorly understood. This study examined ethnic differences in free fatty acids (FFAs) metabolic profile (assessed using liquid chromatography-mass spectrometry), appetite-related hormones and traditional CVD and T2D risk markers in blood samples collected from 16 South Asian and 16 white European men and explored associations with body composition, objectively-measured physical activity and cardiorespiratory fitness. South Asians exhibited higher concentrations of five FFAs (laurate, myristate, palmitate, linolenic, linoleate; p ≤ 0.040), lower acylated ghrelin (ES = 1.00, p = 0.008) and higher leptin (ES = 1.11, p = 0.004) than white Europeans; total peptide YY was similar between groups (p = 0.381). South Asians exhibited elevated fasting insulin, C-reactive protein, interleukin-6, triacylglycerol and ratio of total cholesterol to high-density lipoprotein cholesterol (HDL-C) and lower fasting HDL-C (all ES ≥ 0.74, p ≤ 0.053). Controlling for body fat percentage (assessed using air displacement plethysmography) attenuated these differences. Despite similar habitual moderate-to-vigorous physical activity (ES = 0.18, p = 0.675), V˙ O2max was lower in South Asians (ES = 1.36, p = 0.001). Circulating FFAs in South Asians were positively correlated with body fat percentage (r2 = 0.92), body mass (r2 = 0.86) and AUC glucose (r2 = 0.89) whereas in white Europeans FFAs were negatively correlated with total step counts (r2 = 0.96). In conclusion, South Asians exhibited a different FFA profile, lower ghrelin, higher leptin, impaired CVD and T2D risk markers and lower cardiorespiratory fitness than white Europeans.
LanguageEnglish
Article number71
Number of pages20
JournalMetabolites
Volume9
Issue number4
DOIs
Publication statusPublished - 13 Apr 2019

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Physical Fitness
Metabolome
Adiposity
Appetite
Nonesterified Fatty Acids
Cross-Sectional Studies
Hormones
Exercise
Plasmas
Medical problems
Ghrelin
Type 2 Diabetes Mellitus
Leptin
Cardiovascular Diseases
HDL Cholesterol
Fats
Plethysmography
Laurates
Adipose Tissue
Fasting

Keywords

  • metabolomics
  • free fatty acids
  • cardiovascular disease
  • Type 2 diabetes
  • South Asian
  • physical activity
  • cardiorespiratory fitness
  • inflammation
  • appetite hormones
  • metabolic markers

Cite this

Benedetti, Simone ; Al-Tannak, Naser F. ; Alzharani, Mansour ; Moir, Hannah J. ; Stensel, David J. ; Thackray, Alice E. ; Naughton, Declan P. ; Dorak, Mehmet T. ; Spendif, Owen ; Hill, Natasha ; Watson, David ; Allgrove, Judith. / Plasma free fatty acids metabolic profile with LC-MS and appetite-related hormones in South Asian and white European men in relation to adiposity, physical activity and cardiorespiratory fitness : a cross-sectional study. In: Metabolites. 2019 ; Vol. 9, No. 4.
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Plasma free fatty acids metabolic profile with LC-MS and appetite-related hormones in South Asian and white European men in relation to adiposity, physical activity and cardiorespiratory fitness : a cross-sectional study. / Benedetti, Simone; Al-Tannak, Naser F.; Alzharani, Mansour; Moir, Hannah J.; Stensel, David J.; Thackray, Alice E.; Naughton, Declan P.; Dorak, Mehmet T.; Spendif, Owen; Hill, Natasha; Watson, David; Allgrove, Judith.

In: Metabolites, Vol. 9, No. 4, 71, 13.04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Plasma free fatty acids metabolic profile with LC-MS and appetite-related hormones in South Asian and white European men in relation to adiposity, physical activity and cardiorespiratory fitness

T2 - Metabolites

AU - Benedetti, Simone

AU - Al-Tannak, Naser F.

AU - Alzharani, Mansour

AU - Moir, Hannah J.

AU - Stensel, David J.

AU - Thackray, Alice E.

AU - Naughton, Declan P.

AU - Dorak, Mehmet T.

AU - Spendif, Owen

AU - Hill, Natasha

AU - Watson, David

AU - Allgrove, Judith

PY - 2019/4/13

Y1 - 2019/4/13

N2 - South Asians have a greater cardiovascular disease (CVD) and type 2 diabetes (T2D) risk than white Europeans, but the mechanisms are poorly understood. This study examined ethnic differences in free fatty acids (FFAs) metabolic profile (assessed using liquid chromatography-mass spectrometry), appetite-related hormones and traditional CVD and T2D risk markers in blood samples collected from 16 South Asian and 16 white European men and explored associations with body composition, objectively-measured physical activity and cardiorespiratory fitness. South Asians exhibited higher concentrations of five FFAs (laurate, myristate, palmitate, linolenic, linoleate; p ≤ 0.040), lower acylated ghrelin (ES = 1.00, p = 0.008) and higher leptin (ES = 1.11, p = 0.004) than white Europeans; total peptide YY was similar between groups (p = 0.381). South Asians exhibited elevated fasting insulin, C-reactive protein, interleukin-6, triacylglycerol and ratio of total cholesterol to high-density lipoprotein cholesterol (HDL-C) and lower fasting HDL-C (all ES ≥ 0.74, p ≤ 0.053). Controlling for body fat percentage (assessed using air displacement plethysmography) attenuated these differences. Despite similar habitual moderate-to-vigorous physical activity (ES = 0.18, p = 0.675), V˙ O2max was lower in South Asians (ES = 1.36, p = 0.001). Circulating FFAs in South Asians were positively correlated with body fat percentage (r2 = 0.92), body mass (r2 = 0.86) and AUC glucose (r2 = 0.89) whereas in white Europeans FFAs were negatively correlated with total step counts (r2 = 0.96). In conclusion, South Asians exhibited a different FFA profile, lower ghrelin, higher leptin, impaired CVD and T2D risk markers and lower cardiorespiratory fitness than white Europeans.

AB - South Asians have a greater cardiovascular disease (CVD) and type 2 diabetes (T2D) risk than white Europeans, but the mechanisms are poorly understood. This study examined ethnic differences in free fatty acids (FFAs) metabolic profile (assessed using liquid chromatography-mass spectrometry), appetite-related hormones and traditional CVD and T2D risk markers in blood samples collected from 16 South Asian and 16 white European men and explored associations with body composition, objectively-measured physical activity and cardiorespiratory fitness. South Asians exhibited higher concentrations of five FFAs (laurate, myristate, palmitate, linolenic, linoleate; p ≤ 0.040), lower acylated ghrelin (ES = 1.00, p = 0.008) and higher leptin (ES = 1.11, p = 0.004) than white Europeans; total peptide YY was similar between groups (p = 0.381). South Asians exhibited elevated fasting insulin, C-reactive protein, interleukin-6, triacylglycerol and ratio of total cholesterol to high-density lipoprotein cholesterol (HDL-C) and lower fasting HDL-C (all ES ≥ 0.74, p ≤ 0.053). Controlling for body fat percentage (assessed using air displacement plethysmography) attenuated these differences. Despite similar habitual moderate-to-vigorous physical activity (ES = 0.18, p = 0.675), V˙ O2max was lower in South Asians (ES = 1.36, p = 0.001). Circulating FFAs in South Asians were positively correlated with body fat percentage (r2 = 0.92), body mass (r2 = 0.86) and AUC glucose (r2 = 0.89) whereas in white Europeans FFAs were negatively correlated with total step counts (r2 = 0.96). In conclusion, South Asians exhibited a different FFA profile, lower ghrelin, higher leptin, impaired CVD and T2D risk markers and lower cardiorespiratory fitness than white Europeans.

KW - metabolomics

KW - free fatty acids

KW - cardiovascular disease

KW - Type 2 diabetes

KW - South Asian

KW - physical activity

KW - cardiorespiratory fitness

KW - inflammation

KW - appetite hormones

KW - metabolic markers

UR - https://www.mdpi.com/journal/metabolites

U2 - 10.3390/metabo9040071

DO - 10.3390/metabo9040071

M3 - Article

VL - 9

JO - Metabolites

JF - Metabolites

SN - 2218-1989

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ER -