Phytochemicals from Flemingia vestita (Fabaceae) and Stephania glabra (Menispermeaceae) alter cGMP concentration in the cestode Raillietina echinobothrida

Bidyadhar Das, V. Tandon, L.M. Lyndem, A.I. Gray, V.A. Ferro

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12 Citations (Scopus)

Abstract

Cyclic GMP (cGMP) mediates various physiological functions of nitric oxide (NO) synthesized by nitric oxide synthase (NOS). A crude peel extract and purified fraction of Flemingia vestita, as well as a crude rhizome extract of Stephania glabra and fractions were tested with respect to the activity of NOS, NO efflux and cGMP concentration in the cestode Raillietina echinobothrida in order to find out the possible mode of anthelmintic action of these plant-derived components. For comparison purposes, the parasites were also treated with pure genistein, sodium nitroprusside (SNP-a known NO donor), and the reference drug, praziquantel (PZQ). At the time of onset of paralysis in the parasites, a significant increase (32%-87%) in the NOS activity and a two to three fold increase of NO efflux into the incubation medium were observed in the treated parasites in comparison to their respective controls. The cGMP concentration in the treated parasites' tissue was also increased by 44%-103%. However, in the presence of NG-nitro-L-arginine methyl ester, a potent inhibitor of NOS, there was no increase in the cGMP concentration in the parasite tissue. This study indicates that the phytochemicals, in particular genistein and tetrahydropalmatine, from F. vestita and S. glabra, respectively, disturb the downstream signalling pathway of NO, as indicated by the change in cGMP concentration in the parasite tissue.
LanguageEnglish
Pages397-403
Number of pages7
JournalComparative Biochemistry and Physiology Part C: Toxicology and Pharmacology
Volume149
Issue number3
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Stephania
Cestoda
Cyclic GMP
Phytochemicals
Fabaceae
Parasites
Nitric Oxide Synthase
Nitric Oxide
Genistein
Tissue
Complex Mixtures
Plant Structures
Praziquantel
Rhizome
Anthelmintics
Nitric Oxide Donors
NG-Nitroarginine Methyl Ester
Nitroprusside
Paralysis
Single Nucleotide Polymorphism

Keywords

  • nitric oxide synthase
  • nitric oxide
  • cGMP
  • L-NAME
  • flemingia vestita
  • genistein
  • stephania glabra
  • cestode
  • raillietina echinobothrida

Cite this

@article{f5cdd4a783384affa118e6a98ed5e322,
title = "Phytochemicals from Flemingia vestita (Fabaceae) and Stephania glabra (Menispermeaceae) alter cGMP concentration in the cestode Raillietina echinobothrida",
abstract = "Cyclic GMP (cGMP) mediates various physiological functions of nitric oxide (NO) synthesized by nitric oxide synthase (NOS). A crude peel extract and purified fraction of Flemingia vestita, as well as a crude rhizome extract of Stephania glabra and fractions were tested with respect to the activity of NOS, NO efflux and cGMP concentration in the cestode Raillietina echinobothrida in order to find out the possible mode of anthelmintic action of these plant-derived components. For comparison purposes, the parasites were also treated with pure genistein, sodium nitroprusside (SNP-a known NO donor), and the reference drug, praziquantel (PZQ). At the time of onset of paralysis in the parasites, a significant increase (32{\%}-87{\%}) in the NOS activity and a two to three fold increase of NO efflux into the incubation medium were observed in the treated parasites in comparison to their respective controls. The cGMP concentration in the treated parasites' tissue was also increased by 44{\%}-103{\%}. However, in the presence of NG-nitro-L-arginine methyl ester, a potent inhibitor of NOS, there was no increase in the cGMP concentration in the parasite tissue. This study indicates that the phytochemicals, in particular genistein and tetrahydropalmatine, from F. vestita and S. glabra, respectively, disturb the downstream signalling pathway of NO, as indicated by the change in cGMP concentration in the parasite tissue.",
keywords = "nitric oxide synthase, nitric oxide, cGMP, L-NAME, flemingia vestita, genistein, stephania glabra, cestode, raillietina echinobothrida",
author = "Bidyadhar Das and V. Tandon and L.M. Lyndem and A.I. Gray and V.A. Ferro",
year = "2009",
month = "4",
doi = "10.1016/j.cbpc.2008.09.012",
language = "English",
volume = "149",
pages = "397--403",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology",
issn = "1532-0456",
number = "3",

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TY - JOUR

T1 - Phytochemicals from Flemingia vestita (Fabaceae) and Stephania glabra (Menispermeaceae) alter cGMP concentration in the cestode Raillietina echinobothrida

AU - Das, Bidyadhar

AU - Tandon, V.

AU - Lyndem, L.M.

AU - Gray, A.I.

AU - Ferro, V.A.

PY - 2009/4

Y1 - 2009/4

N2 - Cyclic GMP (cGMP) mediates various physiological functions of nitric oxide (NO) synthesized by nitric oxide synthase (NOS). A crude peel extract and purified fraction of Flemingia vestita, as well as a crude rhizome extract of Stephania glabra and fractions were tested with respect to the activity of NOS, NO efflux and cGMP concentration in the cestode Raillietina echinobothrida in order to find out the possible mode of anthelmintic action of these plant-derived components. For comparison purposes, the parasites were also treated with pure genistein, sodium nitroprusside (SNP-a known NO donor), and the reference drug, praziquantel (PZQ). At the time of onset of paralysis in the parasites, a significant increase (32%-87%) in the NOS activity and a two to three fold increase of NO efflux into the incubation medium were observed in the treated parasites in comparison to their respective controls. The cGMP concentration in the treated parasites' tissue was also increased by 44%-103%. However, in the presence of NG-nitro-L-arginine methyl ester, a potent inhibitor of NOS, there was no increase in the cGMP concentration in the parasite tissue. This study indicates that the phytochemicals, in particular genistein and tetrahydropalmatine, from F. vestita and S. glabra, respectively, disturb the downstream signalling pathway of NO, as indicated by the change in cGMP concentration in the parasite tissue.

AB - Cyclic GMP (cGMP) mediates various physiological functions of nitric oxide (NO) synthesized by nitric oxide synthase (NOS). A crude peel extract and purified fraction of Flemingia vestita, as well as a crude rhizome extract of Stephania glabra and fractions were tested with respect to the activity of NOS, NO efflux and cGMP concentration in the cestode Raillietina echinobothrida in order to find out the possible mode of anthelmintic action of these plant-derived components. For comparison purposes, the parasites were also treated with pure genistein, sodium nitroprusside (SNP-a known NO donor), and the reference drug, praziquantel (PZQ). At the time of onset of paralysis in the parasites, a significant increase (32%-87%) in the NOS activity and a two to three fold increase of NO efflux into the incubation medium were observed in the treated parasites in comparison to their respective controls. The cGMP concentration in the treated parasites' tissue was also increased by 44%-103%. However, in the presence of NG-nitro-L-arginine methyl ester, a potent inhibitor of NOS, there was no increase in the cGMP concentration in the parasite tissue. This study indicates that the phytochemicals, in particular genistein and tetrahydropalmatine, from F. vestita and S. glabra, respectively, disturb the downstream signalling pathway of NO, as indicated by the change in cGMP concentration in the parasite tissue.

KW - nitric oxide synthase

KW - nitric oxide

KW - cGMP

KW - L-NAME

KW - flemingia vestita

KW - genistein

KW - stephania glabra

KW - cestode

KW - raillietina echinobothrida

U2 - 10.1016/j.cbpc.2008.09.012

DO - 10.1016/j.cbpc.2008.09.012

M3 - Article

VL - 149

SP - 397

EP - 403

JO - Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology

T2 - Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology

SN - 1532-0456

IS - 3

ER -