Physiological effects of pathogen reduction of red blood cell products using antimicrobial blue light

Tracy White, Michelle MacLean, Helena Watson, Rachael Tomb, John Anderson, Scott McGregor, Chintamani Atreya

Research output: Contribution to journalMeeting abstract

Abstract

Microbial contamination-associated transfusion-related sepsis though rare, is a significant issue in transfusion medicine. A number of current pathogen reduction technologies utilise ultraviolet or visible light with additional photosensitisers, but have limitations in the treatment of red cell products due to their opacity and sensitivity. Recent advancements have demonstrated the antimicrobial effects of 405-nm blue light for the decontamination of ex vivo animal and human plasma. Lethal oxidative damage occurs through the photo-excitation of endogenous microbial porphyrins at 405-nm, therefore eliminating the need for additional photosensitisers. This research investigated the antimicrobial and physiological effects of 405-nm light on red cell products. Ovine red cell suspensions, seeded with Staphylococcus aureus, were exposed to varying irradiances (1–100 mWcm-2) and doses (21.6-2160 Jcm-2) of 405-nm light to determine germicidal efficiency. Investigation into the effects of the 405-nm light on red cells was also determined through microscopy and hemolysis detection, with further investigation into the potential oxidising effects on hemoglobin through ELISA detection. Results demonstrated successful inactivation of low-level ( < 103 CFUml-1) bacterial contamination in red cell suspensions by 405-nm light treatment, with reduced germicidal potential found upon use of increasing red cell densities. Physiological analysis demonstrated that compatibility with red cell treatment is dependant on the treatment parameters (intensity, treatment time). Low-level illumination showed potential for compatiblity, however, high doses induced varying degrees of hemolysis, Met-hemoglobin formation and reduced viability. Further research is required to fully evaluate the potential of blue light based pathogen reduction in red blood cell products.
Original languageEnglish
Pages (from-to)S58
Number of pages1
JournalExperimental Hematology
Volume53
Issue numbersuppl.
Early online date22 Aug 2017
DOIs
Publication statusPublished - 30 Sep 2017
Event46th Annual Scientific Meeting of the International Society for Experimental Hematology - Goethe University Frankfurt, Frankfurt, Germany
Duration: 24 Aug 201727 Aug 2017

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Pathogens
Blood
Erythrocytes
Cells
Light
Photosensitizing Agents
Photosensitizers
Hemolysis
Hemoglobin
Suspensions
Contamination
Transfusion Medicine
Therapeutics
Methemoglobin
Decontamination
Plasma (human)
Porphyrins
Ultraviolet Rays
Photoexcitation
Lighting

Keywords

  • red blood cells
  • blue light
  • sepsis

Cite this

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title = "Physiological effects of pathogen reduction of red blood cell products using antimicrobial blue light",
abstract = "Microbial contamination-associated transfusion-related sepsis though rare, is a significant issue in transfusion medicine. A number of current pathogen reduction technologies utilise ultraviolet or visible light with additional photosensitisers, but have limitations in the treatment of red cell products due to their opacity and sensitivity. Recent advancements have demonstrated the antimicrobial effects of 405-nm blue light for the decontamination of ex vivo animal and human plasma. Lethal oxidative damage occurs through the photo-excitation of endogenous microbial porphyrins at 405-nm, therefore eliminating the need for additional photosensitisers. This research investigated the antimicrobial and physiological effects of 405-nm light on red cell products. Ovine red cell suspensions, seeded with Staphylococcus aureus, were exposed to varying irradiances (1–100 mWcm-2) and doses (21.6-2160 Jcm-2) of 405-nm light to determine germicidal efficiency. Investigation into the effects of the 405-nm light on red cells was also determined through microscopy and hemolysis detection, with further investigation into the potential oxidising effects on hemoglobin through ELISA detection. Results demonstrated successful inactivation of low-level ( < 103 CFUml-1) bacterial contamination in red cell suspensions by 405-nm light treatment, with reduced germicidal potential found upon use of increasing red cell densities. Physiological analysis demonstrated that compatibility with red cell treatment is dependant on the treatment parameters (intensity, treatment time). Low-level illumination showed potential for compatiblity, however, high doses induced varying degrees of hemolysis, Met-hemoglobin formation and reduced viability. Further research is required to fully evaluate the potential of blue light based pathogen reduction in red blood cell products.",
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Physiological effects of pathogen reduction of red blood cell products using antimicrobial blue light. / White, Tracy; MacLean, Michelle; Watson, Helena; Tomb, Rachael; Anderson, John; McGregor, Scott; Atreya, Chintamani.

In: Experimental Hematology , Vol. 53, No. suppl., 30.09.2017, p. S58.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Physiological effects of pathogen reduction of red blood cell products using antimicrobial blue light

AU - White, Tracy

AU - MacLean, Michelle

AU - Watson, Helena

AU - Tomb, Rachael

AU - Anderson, John

AU - McGregor, Scott

AU - Atreya, Chintamani

PY - 2017/9/30

Y1 - 2017/9/30

N2 - Microbial contamination-associated transfusion-related sepsis though rare, is a significant issue in transfusion medicine. A number of current pathogen reduction technologies utilise ultraviolet or visible light with additional photosensitisers, but have limitations in the treatment of red cell products due to their opacity and sensitivity. Recent advancements have demonstrated the antimicrobial effects of 405-nm blue light for the decontamination of ex vivo animal and human plasma. Lethal oxidative damage occurs through the photo-excitation of endogenous microbial porphyrins at 405-nm, therefore eliminating the need for additional photosensitisers. This research investigated the antimicrobial and physiological effects of 405-nm light on red cell products. Ovine red cell suspensions, seeded with Staphylococcus aureus, were exposed to varying irradiances (1–100 mWcm-2) and doses (21.6-2160 Jcm-2) of 405-nm light to determine germicidal efficiency. Investigation into the effects of the 405-nm light on red cells was also determined through microscopy and hemolysis detection, with further investigation into the potential oxidising effects on hemoglobin through ELISA detection. Results demonstrated successful inactivation of low-level ( < 103 CFUml-1) bacterial contamination in red cell suspensions by 405-nm light treatment, with reduced germicidal potential found upon use of increasing red cell densities. Physiological analysis demonstrated that compatibility with red cell treatment is dependant on the treatment parameters (intensity, treatment time). Low-level illumination showed potential for compatiblity, however, high doses induced varying degrees of hemolysis, Met-hemoglobin formation and reduced viability. Further research is required to fully evaluate the potential of blue light based pathogen reduction in red blood cell products.

AB - Microbial contamination-associated transfusion-related sepsis though rare, is a significant issue in transfusion medicine. A number of current pathogen reduction technologies utilise ultraviolet or visible light with additional photosensitisers, but have limitations in the treatment of red cell products due to their opacity and sensitivity. Recent advancements have demonstrated the antimicrobial effects of 405-nm blue light for the decontamination of ex vivo animal and human plasma. Lethal oxidative damage occurs through the photo-excitation of endogenous microbial porphyrins at 405-nm, therefore eliminating the need for additional photosensitisers. This research investigated the antimicrobial and physiological effects of 405-nm light on red cell products. Ovine red cell suspensions, seeded with Staphylococcus aureus, were exposed to varying irradiances (1–100 mWcm-2) and doses (21.6-2160 Jcm-2) of 405-nm light to determine germicidal efficiency. Investigation into the effects of the 405-nm light on red cells was also determined through microscopy and hemolysis detection, with further investigation into the potential oxidising effects on hemoglobin through ELISA detection. Results demonstrated successful inactivation of low-level ( < 103 CFUml-1) bacterial contamination in red cell suspensions by 405-nm light treatment, with reduced germicidal potential found upon use of increasing red cell densities. Physiological analysis demonstrated that compatibility with red cell treatment is dependant on the treatment parameters (intensity, treatment time). Low-level illumination showed potential for compatiblity, however, high doses induced varying degrees of hemolysis, Met-hemoglobin formation and reduced viability. Further research is required to fully evaluate the potential of blue light based pathogen reduction in red blood cell products.

KW - red blood cells

KW - blue light

KW - sepsis

UR - https://www.sciencedirect.com/science/article/pii/S0301472X17303272

U2 - 10.1016/j.exphem.2017.06.097

DO - 10.1016/j.exphem.2017.06.097

M3 - Meeting abstract

VL - 53

SP - S58

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - suppl.

ER -