Physicochemical characterisation of poly(A) lipid nanoparticles: effect of cryoprotectant and temperature storage conditions

Rand Abdulrahman; Callum G. Davidson; Yvonne Perrie; Kevin Treacher; Catherine Vasey; Robin Capomaccio; Zahra Rattray

Physicochemical characterisation of poly(A) lipid nanoparticles: effect of cryoprotectant and temperature storage conditions

Rand Abdulrahman
, Callum G. Davidson
, Yvonne Perrie
, Kevin Treacher
, Catherine Vasey
, Robin Capomaccio
, Zahra Rattray

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to conference › Poster

199 Downloads (Pure)

Abstract

Lipid nanoparticles (LNPs) are emerging new modalities for mRNA therapeutics which have been in the spotlight for the past decade. Since these are relatively new drug delivery systems compared to conventional medicines, new analytical techniques for the robust characterization of their critical quality attributes (CQAs) are needed [1]. It has been reported that several stimuli can affect the stability of the LNPs such as leakage of the nucleic acid cargo from the nanoparticle and LNP aggregation, resulting in low translation efficiency [2]. Hence, understanding the duration of stability is key during formulation development. The aim of the present study is to evaluate the stability of PolyA-LNPs:
1) Stored at different temperatures (4oC and 25oC); 2) Dialysed in the absence and presence of cryoprotectant sucrose. We measured the impact of the above storage conditions on LNP physicochemical parameters.

Original language	English
Publication status	Published - 21 Jun 2023
Event	European Platform for Technology Network on Nanomedicine 2023 Meeting - University of Liverpool, Liverpool, United Kingdom Duration: 19 Jun 2023 → 22 Jun 2023

Conference

Conference	European Platform for Technology Network on Nanomedicine 2023 Meeting
Country/Territory	United Kingdom
City	Liverpool
Period	19/06/23 → 22/06/23

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

Keywords

nanotechnology
nanomedicine research
lipid nanoparticles
mRNA therapeutics

Access to Document

Abdulrahman-etal-EPTNN-2023-Physicochemical-characterisation-of-poly-A-lipid-nanoparticlesFinal published version, 1.58 MBLicence: CC BY-NC-ND 4.0

Multiscale Metrology Suite for Next-generation Healthcare Technologies (EPSRC Strategic Equipment)
Rattray, Z. (Principal Investigator), Bruns, N. (Co-investigator), Faulds, K. (Co-investigator), Graham, D. (Co-investigator), Halbert, G. (Co-investigator), Hoskins, C. (Co-investigator), McArthur, S. (Co-investigator), Perrie, Y. (Co-investigator), Reid, S. (Co-investigator) & Seib, P. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/11/21 → 31/10/24
Project: Research

FFF
Rattray, Z.
Strathclyde Institute Of Pharmacy And Biomedical Sciences
Facility/equipment: Equipment

Cite this

@conference{b8c3051a19c74cc9bcfe3dcfb0b4cba2,

title = "Physicochemical characterisation of poly(A) lipid nanoparticles: effect of cryoprotectant and temperature storage conditions",

abstract = "Lipid nanoparticles (LNPs) are emerging new modalities for mRNA therapeutics which have been in the spotlight for the past decade. Since these are relatively new drug delivery systems compared to conventional medicines, new analytical techniques for the robust characterization of their critical quality attributes (CQAs) are needed [1]. It has been reported that several stimuli can affect the stability of the LNPs such as leakage of the nucleic acid cargo from the nanoparticle and LNP aggregation, resulting in low translation efficiency [2]. Hence, understanding the duration of stability is key during formulation development. The aim of the present study is to evaluate the stability of PolyA-LNPs: 1) Stored at different temperatures (4oC and 25oC); 2) Dialysed in the absence and presence of cryoprotectant sucrose. We measured the impact of the above storage conditions on LNP physicochemical parameters. ",

keywords = "nanotechnology, nanomedicine research, lipid nanoparticles, mRNA therapeutics",

author = "Rand Abdulrahman and Davidson, \{Callum G.\} and Yvonne Perrie and Kevin Treacher and Catherine Vasey and Robin Capomaccio and Zahra Rattray",

year = "2023",

month = jun,

day = "21",

language = "English",

note = "European Platform for Technology Network on Nanomedicine 2023 Meeting ; Conference date: 19-06-2023 Through 22-06-2023",

}

Physicochemical characterisation of poly(A) lipid nanoparticles: effect of cryoprotectant and temperature storage conditions. / Abdulrahman, Rand ; Davidson, Callum G.; Perrie, Yvonne et al.
2023. Poster session presented at European Platform for Technology Network on Nanomedicine 2023 Meeting, Liverpool, United Kingdom.

Research output: Contribution to conference › Poster

TY - CONF

T1 - Physicochemical characterisation of poly(A) lipid nanoparticles

T2 - European Platform for Technology Network on Nanomedicine 2023 Meeting

AU - Abdulrahman, Rand

AU - Davidson, Callum G.

AU - Perrie, Yvonne

AU - Treacher, Kevin

AU - Vasey, Catherine

AU - Capomaccio, Robin

AU - Rattray, Zahra

PY - 2023/6/21

Y1 - 2023/6/21

N2 - Lipid nanoparticles (LNPs) are emerging new modalities for mRNA therapeutics which have been in the spotlight for the past decade. Since these are relatively new drug delivery systems compared to conventional medicines, new analytical techniques for the robust characterization of their critical quality attributes (CQAs) are needed [1]. It has been reported that several stimuli can affect the stability of the LNPs such as leakage of the nucleic acid cargo from the nanoparticle and LNP aggregation, resulting in low translation efficiency [2]. Hence, understanding the duration of stability is key during formulation development. The aim of the present study is to evaluate the stability of PolyA-LNPs: 1) Stored at different temperatures (4oC and 25oC); 2) Dialysed in the absence and presence of cryoprotectant sucrose. We measured the impact of the above storage conditions on LNP physicochemical parameters.

AB - Lipid nanoparticles (LNPs) are emerging new modalities for mRNA therapeutics which have been in the spotlight for the past decade. Since these are relatively new drug delivery systems compared to conventional medicines, new analytical techniques for the robust characterization of their critical quality attributes (CQAs) are needed [1]. It has been reported that several stimuli can affect the stability of the LNPs such as leakage of the nucleic acid cargo from the nanoparticle and LNP aggregation, resulting in low translation efficiency [2]. Hence, understanding the duration of stability is key during formulation development. The aim of the present study is to evaluate the stability of PolyA-LNPs: 1) Stored at different temperatures (4oC and 25oC); 2) Dialysed in the absence and presence of cryoprotectant sucrose. We measured the impact of the above storage conditions on LNP physicochemical parameters.

KW - nanotechnology

KW - nanomedicine research

KW - lipid nanoparticles

KW - mRNA therapeutics

M3 - Poster

Y2 - 19 June 2023 through 22 June 2023

ER -

Physicochemical characterisation of poly(A) lipid nanoparticles: effect of cryoprotectant and temperature storage conditions

Abstract

Conference

UN SDGs

Keywords

Access to Document

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Projects

Multiscale Metrology Suite for Next-generation Healthcare Technologies (EPSRC Strategic Equipment)

Equipment

FFF

Cite this