Abstract
We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
| Original language | English |
|---|---|
| Pages (from-to) | 4757-4763 |
| Number of pages | 6 |
| Journal | Physics in Medicine and Biology |
| Volume | 49 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2004 |
Keywords
- laser scanning
- microscopy
- photons
- optics
- photonics