Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2

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Abstract

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
Original languageEnglish
Pages (from-to)4757-4763
Number of pages6
JournalPhysics in Medicine and Biology
Volume49
Issue number20
DOIs
Publication statusPublished - 2004

Keywords

  • laser scanning
  • microscopy
  • photons
  • optics
  • photonics

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