Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2

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30 Citations (Scopus)

Abstract

We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
LanguageEnglish
Pages4757-4763
Number of pages6
JournalPhysics in Medicine and Biology
Volume49
Issue number20
DOIs
Publication statusPublished - 2004

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Optics and Photonics
Fura-2
Photons
Fluorescence Microscopy
Confocal Microscopy
Smooth Muscle Myocytes
Pulmonary Veins
Cricetulus
Cardiac Myocytes
Microscopy
Ovary
Lasers
Calcium

Keywords

  • laser scanning
  • microscopy
  • photons
  • optics
  • photonics

Cite this

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abstract = "We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.",
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AB - We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.

KW - laser scanning

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KW - photons

KW - optics

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