Photometry unlocks 3D information from 2D localization microscopy data

Christian Franke, Markus Sauer, Sebastian van de Linde

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)
120 Downloads (Pure)


We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.

Original languageEnglish
Pages (from-to)41-44
Number of pages4
JournalNature Methods
Issue number1
Early online date21 Nov 2016
Publication statusPublished - 31 Jan 2017


  • super resolution
  • microscopy
  • photometry


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