Photometry unlocks 3D information from 2D localization microscopy data

Christian Franke; Markus Sauer; Sebastian van de Linde

doi:10.1038/nmeth.4073

Photometry unlocks 3D information from 2D localization microscopy data

Christian Franke, Markus Sauer, Sebastian van de Linde

Physics

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Abstract

We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.

Original language	English
Pages (from-to)	41-44
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	1
Early online date	21 Nov 2016
DOIs	https://doi.org/10.1038/nmeth.4073
Publication status	Published - 31 Jan 2017

Keywords

super resolution
microscopy
photometry

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10.1038/nmeth.4073

Franke-etal-NM-2017-Photometry-unlocks-3D-information-from-2D-localization-microscopyAccepted author manuscript, 176 KB

Cite this

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Photometry unlocks 3D information from 2D localization microscopy data

Abstract

Keywords

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