Photometry unlocks 3D information from 2D localization microscopy data

Christian Franke, Markus Sauer, Sebastian van de Linde

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.

LanguageEnglish
Pages41-44
Number of pages4
JournalNature Methods
Volume14
Issue number1
Early online date21 Nov 2016
DOIs
Publication statusPublished - 31 Jan 2017

Fingerprint

Photometry
Microscopy
Microscopic examination
Fluorophores
Photons

Keywords

  • super resolution
  • microscopy
  • photometry

Cite this

Franke, Christian ; Sauer, Markus ; van de Linde, Sebastian. / Photometry unlocks 3D information from 2D localization microscopy data. In: Nature Methods . 2017 ; Vol. 14, No. 1. pp. 41-44.
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Photometry unlocks 3D information from 2D localization microscopy data. / Franke, Christian; Sauer, Markus; van de Linde, Sebastian.

In: Nature Methods , Vol. 14, No. 1, 31.01.2017, p. 41-44.

Research output: Contribution to journalArticle

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