Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise

C. Karatzaferi, A. De Haan, R.A. Ferguson, W. Van Mechelen, A.J. Sargeant

Research output: Contribution to journalArticle

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Abstract

The recovery of high-energy phosphate levels in single human skeletal muscle fibres following short-term maximal (all-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately post-exercise and at 1.5 min of recovery. The subjects also performed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fibres were analysed for adenosine 5'-triphosphate (ATP), inosine-5'-monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Cr content than type II fibres (P<0.01). Within type II fibres resting [PCr] increased with increasing MyHC IIX isoform content (r=0.59, P<0.01). Post-exercise [PCr] was very low in all fibre groups (P<0.01 versus rest) while great reductions in ATP were also observed (P<0.01 versus rest), especially in the type II fibre groups. [PCr] at 1.5 min of recovery was still lower compared to rest for all fibre groups (P<0.01) especially in the IIAx and IIXa fibres.
LanguageEnglish
Pages467-474
Number of pages8
JournalPflugers Archiv - European Journal of Physiology
Volume442
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Phosphocreatine
Adenosine
Muscle
Myosin Heavy Chains
Adenosine Triphosphate
Exercise
Muscles
Creatine
Fibers
Protein Isoforms
Inosine Monophosphate
Skeletal Muscle Fibers
Quadriceps Muscle
Volunteers
Recovery
Phosphates
Biopsy
triphosphoric acid
Inosine Triphosphate
Exercise equipment

Keywords

  • phosphocreatine
  • muscle fibres
  • phosphate
  • muscle biopsy
  • exercise

Cite this

Karatzaferi, C. ; De Haan, A. ; Ferguson, R.A. ; Van Mechelen, W. ; Sargeant, A.J. / Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise. In: Pflugers Archiv - European Journal of Physiology. 2001 ; Vol. 442, No. 3. pp. 467-474.
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abstract = "The recovery of high-energy phosphate levels in single human skeletal muscle fibres following short-term maximal (all-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately post-exercise and at 1.5 min of recovery. The subjects also performed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50{\%} IIX MyHC). Fibres were analysed for adenosine 5'-triphosphate (ATP), inosine-5'-monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Cr content than type II fibres (P<0.01). Within type II fibres resting [PCr] increased with increasing MyHC IIX isoform content (r=0.59, P<0.01). Post-exercise [PCr] was very low in all fibre groups (P<0.01 versus rest) while great reductions in ATP were also observed (P<0.01 versus rest), especially in the type II fibre groups. [PCr] at 1.5 min of recovery was still lower compared to rest for all fibre groups (P<0.01) especially in the IIAx and IIXa fibres.",
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Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise. / Karatzaferi, C.; De Haan, A.; Ferguson, R.A.; Van Mechelen, W.; Sargeant, A.J.

In: Pflugers Archiv - European Journal of Physiology, Vol. 442, No. 3, 2001, p. 467-474.

Research output: Contribution to journalArticle

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T1 - Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise

AU - Karatzaferi, C.

AU - De Haan, A.

AU - Ferguson, R.A.

AU - Van Mechelen, W.

AU - Sargeant, A.J.

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AB - The recovery of high-energy phosphate levels in single human skeletal muscle fibres following short-term maximal (all-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately post-exercise and at 1.5 min of recovery. The subjects also performed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fibres were analysed for adenosine 5'-triphosphate (ATP), inosine-5'-monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Cr content than type II fibres (P<0.01). Within type II fibres resting [PCr] increased with increasing MyHC IIX isoform content (r=0.59, P<0.01). Post-exercise [PCr] was very low in all fibre groups (P<0.01 versus rest) while great reductions in ATP were also observed (P<0.01 versus rest), especially in the type II fibre groups. [PCr] at 1.5 min of recovery was still lower compared to rest for all fibre groups (P<0.01) especially in the IIAx and IIXa fibres.

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