PH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery

Anja Car, Patric Baumann, Jason T. Duskey, Mohamed Chami, Nico Bruns, Wolfgang Meier

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A series of poly(dimethysiloxane)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMS-b-PDMAEMA) block copolymers were synthesized with atom transfer radical polymerization (ATRP). In aqueous solution the polymers self-assembled into micelles with diameters between 80 and 300 nm, with the ability to encapsulate DOX. The polymer with the shortest PDMAEMA block (5 units) displayed excellent cell viability, while micelles containing longer PDMAEMA block lengths (13 and 22 units) led to increased cytotoxicity. The carriers released DOX in response to a decrease in pH from 7.4 to 5.5. Confocal laser scanning microscopy (CLSM) revealed that nanoparticles were taken up by endocytosis into acidic cell compartments. Furthermore, DOX-loaded nanocarriers exhibited intracellular pH-response as changes in cell morphology and drug release were observed within 24 h.

LanguageEnglish
Pages3235-3245
Number of pages11
JournalBiomacromolecules
Volume15
Issue number9
DOIs
Publication statusPublished - 8 Sep 2014

Fingerprint

Micelles
Drug delivery
Atom transfer radical polymerization
Polymers
Cytotoxicity
Block copolymers
Microscopic examination
Cells
Nanoparticles
Scanning
Lasers
Pharmaceutical Preparations
poly(2-(dimethylamino)ethyl methacrylate)

Keywords

  • amphiphilic block copolymers
  • nanoparticles
  • drug discovery

Cite this

Car, Anja ; Baumann, Patric ; Duskey, Jason T. ; Chami, Mohamed ; Bruns, Nico ; Meier, Wolfgang. / PH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery. In: Biomacromolecules. 2014 ; Vol. 15, No. 9. pp. 3235-3245.
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abstract = "A series of poly(dimethysiloxane)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMS-b-PDMAEMA) block copolymers were synthesized with atom transfer radical polymerization (ATRP). In aqueous solution the polymers self-assembled into micelles with diameters between 80 and 300 nm, with the ability to encapsulate DOX. The polymer with the shortest PDMAEMA block (5 units) displayed excellent cell viability, while micelles containing longer PDMAEMA block lengths (13 and 22 units) led to increased cytotoxicity. The carriers released DOX in response to a decrease in pH from 7.4 to 5.5. Confocal laser scanning microscopy (CLSM) revealed that nanoparticles were taken up by endocytosis into acidic cell compartments. Furthermore, DOX-loaded nanocarriers exhibited intracellular pH-response as changes in cell morphology and drug release were observed within 24 h.",
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Car, A, Baumann, P, Duskey, JT, Chami, M, Bruns, N & Meier, W 2014, 'PH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery' Biomacromolecules, vol. 15, no. 9, pp. 3235-3245. https://doi.org/10.1021/bm500919z

PH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery. / Car, Anja; Baumann, Patric; Duskey, Jason T.; Chami, Mohamed; Bruns, Nico; Meier, Wolfgang.

In: Biomacromolecules, Vol. 15, No. 9, 08.09.2014, p. 3235-3245.

Research output: Contribution to journalArticle

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T1 - PH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery

AU - Car, Anja

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AU - Meier, Wolfgang

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