Abstract
| Language | English |
|---|---|
| Pages | 211-220 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research Part A |
| Volume | 99A |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 2011 |
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Keywords
- functional alginate
- tissue engineering
- endometrium
- fibronectin
- perfusion microbioreactor
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Perfusion culture enhanced human endometrial stromal cell growth in alginate-multivalent integrin α5β1 ligand scaffolds. / Li, Zhaohui; Kreiner, Michaela; Edrada-Ebel, Ruangelie; Cui, Zhanfeng; van der Walle, Christopher F; Mardon, Helen J.
In: Journal of Biomedical Materials Research Part A, Vol. 99A, No. 2, 11.2011, p. 211-220.Research output: Contribution to journal › Article
TY - JOUR
T1 - Perfusion culture enhanced human endometrial stromal cell growth in alginate-multivalent integrin α5β1 ligand scaffolds
AU - Li, Zhaohui
AU - Kreiner, Michaela
AU - Edrada-Ebel, Ruangelie
AU - Cui, Zhanfeng
AU - van der Walle, Christopher F
AU - Mardon, Helen J
N1 - Copyright © 2011 Wiley Periodicals, Inc.
PY - 2011/11
Y1 - 2011/11
N2 - A method to functionalize alginate by introducing monomeric or self-assembling (tetrameric) fibronectin (FN) domains is described, leading to a functional scaffold, which is used for three dimensional (3D) culture of human endometrial stromal cells (EnSCs). EnSCs encapsulated in the functional alginate were cultured under perfusion using the TissueFlex® platform, a multiple parallel microbioreactor system for 3D cell culture. The effect of the novel scaffold and the effect of perfusion were examined. Cell viability, proliferation, and extracellular matrix (ECM) deposition were determined and the results compared with those obtained with cells encapsulated in non-functionalized alginate, and also those without perfusion. Staining for focal adhesions and actin showed maximal cell adhesion only for alginate-tetrameric FN scaffolds under perfusion, associated with a significant increase in cell number over 7 days culture; in contrast to poor cell adhesion and a decrease in cell number for non-functionalized alginate scaffolds (irrespective of perfused/static culture) and 3D static culture (irrespective of the scaffold). Conjugation of alginate to FN was an absolute requirement to attenuate the loss of cell metabolic activity over 7 days culture. ECM deposition for blank alginate and alginate-monomeric FN was similar, but increased around 2-fold and 3-fold for alginate-tetrameric FN under static and perfusion culture, respectively. It is concluded that the requirement for EnSC engagement with multivalent integrin α5β1 ligands and perfused culture are both essential as a first step toward endometrial tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
AB - A method to functionalize alginate by introducing monomeric or self-assembling (tetrameric) fibronectin (FN) domains is described, leading to a functional scaffold, which is used for three dimensional (3D) culture of human endometrial stromal cells (EnSCs). EnSCs encapsulated in the functional alginate were cultured under perfusion using the TissueFlex® platform, a multiple parallel microbioreactor system for 3D cell culture. The effect of the novel scaffold and the effect of perfusion were examined. Cell viability, proliferation, and extracellular matrix (ECM) deposition were determined and the results compared with those obtained with cells encapsulated in non-functionalized alginate, and also those without perfusion. Staining for focal adhesions and actin showed maximal cell adhesion only for alginate-tetrameric FN scaffolds under perfusion, associated with a significant increase in cell number over 7 days culture; in contrast to poor cell adhesion and a decrease in cell number for non-functionalized alginate scaffolds (irrespective of perfused/static culture) and 3D static culture (irrespective of the scaffold). Conjugation of alginate to FN was an absolute requirement to attenuate the loss of cell metabolic activity over 7 days culture. ECM deposition for blank alginate and alginate-monomeric FN was similar, but increased around 2-fold and 3-fold for alginate-tetrameric FN under static and perfusion culture, respectively. It is concluded that the requirement for EnSC engagement with multivalent integrin α5β1 ligands and perfused culture are both essential as a first step toward endometrial tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
KW - functional alginate
KW - tissue engineering
KW - endometrium
KW - fibronectin
KW - perfusion microbioreactor
UR - http://www.scopus.com/inward/record.url?scp=80053183458&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.33177
DO - 10.1002/jbm.a.33177
M3 - Article
VL - 99A
SP - 211
EP - 220
JO - Journal of Biomedical Materials Research Part A
T2 - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
SN - 1549-3296
IS - 2
ER -