Peptide nanofibers with dynamic instability through non-equilibrium biocatalytic assembly

Sisir Debnath, Sangita Roy, Rein Ulijn

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We demonstrate supramolecular peptide nanofibers that display dynamic instability, i.e. they are formed by competing assembly and dis-assembly reactions, where assembly is favoured away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide derivative from
amino acid precursors, that temporarily exceeds the critical gelation concentration, until the
competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows
consecutive nanofiber formation and shortening. The process results in macroscopically
observable temporary hydrogelation, which may be repeated upon refuelling the system
with further addition of the chemically activated amino acid pre-cursor. Non-equilibrium
nanostructures open up opportunities for mimicry of the behaviour dynamic gels found in
natural systems and provide components for future adaptive nanotechnologies.
LanguageEnglish
Pages16789-16792
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number45
Early online date22 Oct 2013
DOIs
Publication statusPublished - 13 Nov 2013

Fingerprint

Nanofibers
Peptides
Nanotechnology
Atomic Force Microscopy
Hydrolysis
Gels
Gelation
Amino Acids
Acids
Amino acids
Atomic force microscopy
Derivatives

Keywords

  • peptide nanofibres
  • biocatalytic self-assembly
  • non-equilibrium

Cite this

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Peptide nanofibers with dynamic instability through non-equilibrium biocatalytic assembly. / Debnath, Sisir; Roy, Sangita; Ulijn, Rein.

In: Journal of the American Chemical Society, Vol. 135 , No. 45, 13.11.2013, p. 16789-16792.

Research output: Contribution to journalArticle

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