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Abstract
Silk fibroin nanoparticles are emerging as promising nanomedicines, but their full therapeutic potential is yet to be realized. These nanoparticles can be readily PEGylated to improve colloidal stability and to tune degradation and drug release profiles; however, the relationship between silk fibroin nanoparticle PEGylation and macrophage activation still requires elucidation. Here, we used in vitro assays and nuclear magnetic resonance based metabolomics to examine the inflammatory phenotype and metabolic profiles of macrophages following their exposure to unmodified or PEGylated silk fibroin nanoparticles. The macrophages internalized both types of nanoparticles, but they showed different phenotypic and metabolic responses to each nanoparticle type. Unmodified silk fibroin nanoparticles induced the upregulation of several processes, including production of proinflammatory mediators (e.g., cytokines), release of nitric oxide, and promotion of antioxidant activity. These responses were accompanied by changes in the macrophage metabolomic profiles that were consistent with a proinflammatory state and that indicated an increase in glycolysis and reprogramming of the tricarboxylic acid cycle and the creatine kinase/phosphocreatine pathway. By contrast, PEGylated silk fibroin nanoparticles induced milder changes to both inflammatory and metabolic profiles, suggesting that immunomodulation of macrophages with silk fibroin nanoparticles is PEGylation-dependent. Overall, PEGylation of silk fibroin nanoparticles reduced the inflammatory and metabolic responses initiated by macrophages, and this observation could be used to guide the therapeutic applications of these nanoparticles.
Original language | English |
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Pages (from-to) | 14515-14525 |
Number of pages | 11 |
Journal | ACS Applied Materials and Interfaces |
Volume | 11 |
Issue number | 16 |
Early online date | 12 Apr 2019 |
DOIs | |
Publication status | Published - 24 Apr 2019 |
Keywords
- silk
- fibroin
- silk nanoparticles
- NMR metabolomics
- macrophages
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Philipp Seib
- SULSA
- Strathclyde Institute Of Pharmacy And Biomedical Sciences - Visiting Professor
Person: Academic, Visiting Professor
Projects
- 1 Finished
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Doctoral Training Partnership (DTP - University of Strathclyde)
McFarlane, A. (Principal Investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/15 → 30/09/19
Project: Research - Studentship
Datasets
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Data for: "PEGylation-dependent metabolic rewiring of macrophages with silk fibroin nanoparticles"
Totten, J. (Creator), Seib, P. (Creator), Wongpinyochit, T. (Creator), Duarte, I. (Creator) & Carrola, J. (Creator), University of Strathclyde, 28 Mar 2019
DOI: 10.15129/f03c40f2-1eef-44a0-891a-628abbe5cb16
Dataset