Parasites lacking the micronemal protein MIC2 are deficient in surface attachment and host cell egress, but remain virulent in vivo

Simon Gras, Allison Jackson, Stuart Woods, Gurnman Pall, Jamie Whitelaw, Jacqueline M. Leung, Gary E. Ward, Craig W. Roberts, Markus Meissner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Micronemal proteins of the thrombospondin-related anonymous protein (TRAP) family are believed to play essential roles during gliding motility and host cell invasion by apicomplexan parasites, and currently represent major vaccine candidates against Plasmodium falciparum, the causative agent of malaria. However, recent evidence suggests that they play multiple and different roles than previously assumed. Here, we analyse a null mutant for MIC2, the TRAP homolog in Toxoplasma gondii. Methods: We performed a careful analysis of parasite motility in a 3D-environment, attachment under shear stress conditions, host cell invasion and in vivo virulence. Results: We verified the role of MIC2 in efficient surface attachment, but were unable to identify any direct function of MIC2 in sustaining gliding motility or host cell invasion once initiated. Furthermore, we find that deletion of mic2 causes a slightly delayed infection in vivo, leading only to mild attenuation of virulence; like with wildtype parasites, inoculation with even low numbers of mic2 KO parasites causes lethal disease in mice. However, deletion of mic2 causes delayed host cell egress in vitro, possibly via disrupted signal transduction pathways. Conclusions: We confirm a critical role of MIC2 in parasite attachment to the surface, leading to reduced parasite motility and host cell invasion. However, MIC2 appears to not be critical for gliding motility or host cell invasion, since parasite speed during these processes is unaffected. Furthermore, deletion of MIC2 leads only to slight attenuation of the parasite.
LanguageEnglish
Pages1
Number of pages15
JournalWellcome Open Research
Volume2
Issue number32
DOIs
Publication statusPublished - 19 May 2017

Fingerprint

Parasites
Proteins
Thrombospondins
Cell Movement
Virulence
Signal transduction
CD99 Antigen
Toxoplasma
Plasmodium falciparum
Malaria
Shear stress
Signal Transduction
Vaccines
Infection

Keywords

  • micronemal proteins
  • thrombospondin-related anonymous protein
  • gliding motility
  • host cell invasion
  • apicomplexan parasite
  • Toxoplasma gondii
  • parasite motility
  • in vivo virulence

Cite this

Gras, Simon ; Jackson, Allison ; Woods, Stuart ; Pall, Gurnman ; Whitelaw, Jamie ; Leung, Jacqueline M. ; Ward, Gary E. ; Roberts, Craig W. ; Meissner, Markus. / Parasites lacking the micronemal protein MIC2 are deficient in surface attachment and host cell egress, but remain virulent in vivo. In: Wellcome Open Research. 2017 ; Vol. 2, No. 32. pp. 1.
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abstract = "Background: Micronemal proteins of the thrombospondin-related anonymous protein (TRAP) family are believed to play essential roles during gliding motility and host cell invasion by apicomplexan parasites, and currently represent major vaccine candidates against Plasmodium falciparum, the causative agent of malaria. However, recent evidence suggests that they play multiple and different roles than previously assumed. Here, we analyse a null mutant for MIC2, the TRAP homolog in Toxoplasma gondii. Methods: We performed a careful analysis of parasite motility in a 3D-environment, attachment under shear stress conditions, host cell invasion and in vivo virulence. Results: We verified the role of MIC2 in efficient surface attachment, but were unable to identify any direct function of MIC2 in sustaining gliding motility or host cell invasion once initiated. Furthermore, we find that deletion of mic2 causes a slightly delayed infection in vivo, leading only to mild attenuation of virulence; like with wildtype parasites, inoculation with even low numbers of mic2 KO parasites causes lethal disease in mice. However, deletion of mic2 causes delayed host cell egress in vitro, possibly via disrupted signal transduction pathways. Conclusions: We confirm a critical role of MIC2 in parasite attachment to the surface, leading to reduced parasite motility and host cell invasion. However, MIC2 appears to not be critical for gliding motility or host cell invasion, since parasite speed during these processes is unaffected. Furthermore, deletion of MIC2 leads only to slight attenuation of the parasite.",
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Parasites lacking the micronemal protein MIC2 are deficient in surface attachment and host cell egress, but remain virulent in vivo. / Gras, Simon; Jackson, Allison; Woods, Stuart; Pall, Gurnman; Whitelaw, Jamie; Leung, Jacqueline M.; Ward, Gary E.; Roberts, Craig W.; Meissner, Markus.

In: Wellcome Open Research, Vol. 2, No. 32, 19.05.2017, p. 1.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Parasites lacking the micronemal protein MIC2 are deficient in surface attachment and host cell egress, but remain virulent in vivo

AU - Gras, Simon

AU - Jackson, Allison

AU - Woods, Stuart

AU - Pall, Gurnman

AU - Whitelaw, Jamie

AU - Leung, Jacqueline M.

AU - Ward, Gary E.

AU - Roberts, Craig W.

AU - Meissner, Markus

PY - 2017/5/19

Y1 - 2017/5/19

N2 - Background: Micronemal proteins of the thrombospondin-related anonymous protein (TRAP) family are believed to play essential roles during gliding motility and host cell invasion by apicomplexan parasites, and currently represent major vaccine candidates against Plasmodium falciparum, the causative agent of malaria. However, recent evidence suggests that they play multiple and different roles than previously assumed. Here, we analyse a null mutant for MIC2, the TRAP homolog in Toxoplasma gondii. Methods: We performed a careful analysis of parasite motility in a 3D-environment, attachment under shear stress conditions, host cell invasion and in vivo virulence. Results: We verified the role of MIC2 in efficient surface attachment, but were unable to identify any direct function of MIC2 in sustaining gliding motility or host cell invasion once initiated. Furthermore, we find that deletion of mic2 causes a slightly delayed infection in vivo, leading only to mild attenuation of virulence; like with wildtype parasites, inoculation with even low numbers of mic2 KO parasites causes lethal disease in mice. However, deletion of mic2 causes delayed host cell egress in vitro, possibly via disrupted signal transduction pathways. Conclusions: We confirm a critical role of MIC2 in parasite attachment to the surface, leading to reduced parasite motility and host cell invasion. However, MIC2 appears to not be critical for gliding motility or host cell invasion, since parasite speed during these processes is unaffected. Furthermore, deletion of MIC2 leads only to slight attenuation of the parasite.

AB - Background: Micronemal proteins of the thrombospondin-related anonymous protein (TRAP) family are believed to play essential roles during gliding motility and host cell invasion by apicomplexan parasites, and currently represent major vaccine candidates against Plasmodium falciparum, the causative agent of malaria. However, recent evidence suggests that they play multiple and different roles than previously assumed. Here, we analyse a null mutant for MIC2, the TRAP homolog in Toxoplasma gondii. Methods: We performed a careful analysis of parasite motility in a 3D-environment, attachment under shear stress conditions, host cell invasion and in vivo virulence. Results: We verified the role of MIC2 in efficient surface attachment, but were unable to identify any direct function of MIC2 in sustaining gliding motility or host cell invasion once initiated. Furthermore, we find that deletion of mic2 causes a slightly delayed infection in vivo, leading only to mild attenuation of virulence; like with wildtype parasites, inoculation with even low numbers of mic2 KO parasites causes lethal disease in mice. However, deletion of mic2 causes delayed host cell egress in vitro, possibly via disrupted signal transduction pathways. Conclusions: We confirm a critical role of MIC2 in parasite attachment to the surface, leading to reduced parasite motility and host cell invasion. However, MIC2 appears to not be critical for gliding motility or host cell invasion, since parasite speed during these processes is unaffected. Furthermore, deletion of MIC2 leads only to slight attenuation of the parasite.

KW - micronemal proteins

KW - thrombospondin-related anonymous protein

KW - gliding motility

KW - host cell invasion

KW - apicomplexan parasite

KW - Toxoplasma gondii

KW - parasite motility

KW - in vivo virulence

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DO - 10.12688/wellcomeopenres.11594.1

M3 - Article

VL - 2

SP - 1

JO - Wellcome Open Research

T2 - Wellcome Open Research

JF - Wellcome Open Research

SN - 2398-502X

IS - 32

ER -