Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation

Craig Russell, Sumyra Begum, Yasar Hussain, Majad Hussain, David Huen, Ayesha S. Rahman, Yvonne Perrie, Afzal R. Mohammed

Research output: Contribution to journalArticle

Abstract

Ramipril is used mainly for the treatment of hypertension and to reduce incidence of fatality following heart attacks in patients who develop indications of congestive heart failure. In the paediatric population, it is used most commonly for the treatment of heart failure, hypertension in type 1 diabetes and diabetic nephropathy. Due to the lack of a suitable liquid formulation, the current study evaluates the development of a range of oral liquid formulations of ramipril along with their in vitro and in vivo absorption studies. Three different formulation development approaches were studied: solubilisation using acetic acid as a co-solvent, complexation with hydroxypropyl-β-cyclodextrin (HP-β-CD) and suspension development using xanthan gum. Systematic optimisation of formulation parameters for the different strategies resulted in the development of products stable for 12 months at long-term stability conditions. In vivo evaluation showed C(max) of 10.48 µg/ml for co-solvent, 13.04 µg/ml for the suspension and 29.58 µg/ml for the cyclodextrin-based ramipril solution. Interestingly, both ramipril solution (co-solvent) and the suspension showed a T(max) of 2.5 h, however, cyclodextrin-based ramipril produced T(max) at 0.75 h following administration. The results presented in this study provide translatable products for oral liquid ramipril which offer preferential paediatric use over existing alternatives.

LanguageEnglish
Pages854-863
Number of pages10
JournalJournal of Drug Targeting
Volume23
Issue number9
Early online date7 May 2015
DOIs
Publication statusPublished - 7 May 2015

Fingerprint

Ramipril
Pediatrics
Pharmaceutical Preparations
Suspensions
Cyclodextrins
Heart Failure
Hypertension
Diabetic Nephropathies
Treatment Failure
Type 1 Diabetes Mellitus
Acetic Acid
In Vitro Techniques
Myocardial Infarction
Incidence
Population

Keywords

  • Caco2
  • liquid formulation
  • oral drug delivery
  • ramipril

Cite this

Russell, C., Begum, S., Hussain, Y., Hussain, M., Huen, D., Rahman, A. S., ... Mohammed, A. R. (2015). Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation. Journal of Drug Targeting, 23(9), 854-863. https://doi.org/10.3109/1061186X.2015.1036275
Russell, Craig ; Begum, Sumyra ; Hussain, Yasar ; Hussain, Majad ; Huen, David ; Rahman, Ayesha S. ; Perrie, Yvonne ; Mohammed, Afzal R. / Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation. In: Journal of Drug Targeting. 2015 ; Vol. 23, No. 9. pp. 854-863.
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Russell, C, Begum, S, Hussain, Y, Hussain, M, Huen, D, Rahman, AS, Perrie, Y & Mohammed, AR 2015, 'Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation' Journal of Drug Targeting, vol. 23, no. 9, pp. 854-863. https://doi.org/10.3109/1061186X.2015.1036275

Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation. / Russell, Craig; Begum, Sumyra; Hussain, Yasar; Hussain, Majad; Huen, David; Rahman, Ayesha S.; Perrie, Yvonne; Mohammed, Afzal R.

In: Journal of Drug Targeting, Vol. 23, No. 9, 07.05.2015, p. 854-863.

Research output: Contribution to journalArticle

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T1 - Paediatric drug development of ramipril reformulation, in vitro and in vivo evaluation

AU - Russell, Craig

AU - Begum, Sumyra

AU - Hussain, Yasar

AU - Hussain, Majad

AU - Huen, David

AU - Rahman, Ayesha S.

AU - Perrie, Yvonne

AU - Mohammed, Afzal R.

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