P43 The isolation and purification of bioactive metabolites from Ficus carica and their neuroprotective effects in Alzheimer's disease

Hanan Khojah, RuAngelie Edrada-Ebel

Research output: Contribution to journalMeeting abstract

Abstract

Neurodegenerative disease including Alzheimer’s disease (AD) is a major cause of long-term disability. Incidence of Alzheimer’s disease increases with age. According to a recent report by WHO, there are approximately 47.5 million people suffering from dementia, with 7.7 million new cases each year. Acrolein is one of the key contributing factors to accelerate oxidative stress which then implicated AD. However, protection against neuronal damage remains a great challenge for researchers. Ficus carica (commonly known as fig) is a species of great antioxidant nutritional value comprising a protective mechanism against innumerable health disorders related to oxidative stress as well as AD. The purpose of this work was to characterise the bioactive metabolites in Ficus carica mesocarp against oxidative stress related AD. Crude extracts were prepared using several extraction methods to achieve non-polar layer. The isolated metabolites were tested on their effect on human neuroblastoma SHSY5Y cell line by a cell viability test. Molecular weights of the active metabolites were determined via LC-HRESIMS, GC-EIMS, and multivariate analysis via SIMCA was performed. Structural elucidation and identification of the interested active metabolites were studied by 1-D and 2-D NMR. NMR profiling demonstrated that aliphatic compounds such as γ-sitosterol were found to induce neuronal bioactivity.
LanguageEnglish
Pages140
Number of pages1
JournalBiochemical Pharmacology
Volume139
Issue numberSupplement C
DOIs
StatePublished - 1 Sep 2017
EventICMAN / IUPHAR Conference 2017 - Aberdeen, United Kingdom
Duration: 27 Sep 201729 Nov 2017

Fingerprint

Carica
Ficus
Neuroprotective Agents
Metabolites
Purification
Alzheimer Disease
Oxidative stress
Oxidative Stress
Cells
Nuclear magnetic resonance
Neurodegenerative diseases
Acrolein
Nutritive Value
Bioactivity
Complex Mixtures
Neuroblastoma
Neurodegenerative Diseases
Dementia
Cell Survival
Multivariate Analysis

Keywords

  • Alzheimer's disease
  • neurodegenerative diseases
  • Ficus carica
  • SHSY5Y
  • LC-HRESIMS
  • GC-EIMS
  • neuronal bioactivity

Cite this

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title = "P43 The isolation and purification of bioactive metabolites from Ficus carica and their neuroprotective effects in Alzheimer's disease",
abstract = "Neurodegenerative disease including Alzheimer’s disease (AD) is a major cause of long-term disability. Incidence of Alzheimer’s disease increases with age. According to a recent report by WHO, there are approximately 47.5 million people suffering from dementia, with 7.7 million new cases each year. Acrolein is one of the key contributing factors to accelerate oxidative stress which then implicated AD. However, protection against neuronal damage remains a great challenge for researchers. Ficus carica (commonly known as fig) is a species of great antioxidant nutritional value comprising a protective mechanism against innumerable health disorders related to oxidative stress as well as AD. The purpose of this work was to characterise the bioactive metabolites in Ficus carica mesocarp against oxidative stress related AD. Crude extracts were prepared using several extraction methods to achieve non-polar layer. The isolated metabolites were tested on their effect on human neuroblastoma SHSY5Y cell line by a cell viability test. Molecular weights of the active metabolites were determined via LC-HRESIMS, GC-EIMS, and multivariate analysis via SIMCA was performed. Structural elucidation and identification of the interested active metabolites were studied by 1-D and 2-D NMR. NMR profiling demonstrated that aliphatic compounds such as γ-sitosterol were found to induce neuronal bioactivity.",
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author = "Hanan Khojah and RuAngelie Edrada-Ebel",
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P43 The isolation and purification of bioactive metabolites from Ficus carica and their neuroprotective effects in Alzheimer's disease. / Khojah, Hanan; Edrada-Ebel, RuAngelie.

In: Biochemical Pharmacology, Vol. 139, No. Supplement C, 01.09.2017, p. 140.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - P43 The isolation and purification of bioactive metabolites from Ficus carica and their neuroprotective effects in Alzheimer's disease

AU - Khojah,Hanan

AU - Edrada-Ebel,RuAngelie

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Y1 - 2017/9/1

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AB - Neurodegenerative disease including Alzheimer’s disease (AD) is a major cause of long-term disability. Incidence of Alzheimer’s disease increases with age. According to a recent report by WHO, there are approximately 47.5 million people suffering from dementia, with 7.7 million new cases each year. Acrolein is one of the key contributing factors to accelerate oxidative stress which then implicated AD. However, protection against neuronal damage remains a great challenge for researchers. Ficus carica (commonly known as fig) is a species of great antioxidant nutritional value comprising a protective mechanism against innumerable health disorders related to oxidative stress as well as AD. The purpose of this work was to characterise the bioactive metabolites in Ficus carica mesocarp against oxidative stress related AD. Crude extracts were prepared using several extraction methods to achieve non-polar layer. The isolated metabolites were tested on their effect on human neuroblastoma SHSY5Y cell line by a cell viability test. Molecular weights of the active metabolites were determined via LC-HRESIMS, GC-EIMS, and multivariate analysis via SIMCA was performed. Structural elucidation and identification of the interested active metabolites were studied by 1-D and 2-D NMR. NMR profiling demonstrated that aliphatic compounds such as γ-sitosterol were found to induce neuronal bioactivity.

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