Oxygen transport and cell viability in an annular flow bioreactor: comparison of laminar Couette and Taylor-vortex flow regimes

S.J. Curran, R.A. Black

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Rotating wall vessel bioreactors have been proposed as a means of controlling the fluid dynamic environment during long-term culture of mammalian cells and engineered tissues. In this study, we show how the delivery of oxygen to cells in an annular flow bioreactor is enhanced by the forced convective transport afforded by Taylor vortex flows. A fiberoptic oxygen probe with negligible lag time was used to measure the dissolved oxygen concentration in real time and under carefully controlled aeration conditions. From these data, the overall mass transfer coefficients were calculated and mass transport correlations determined under laminar Couette flow conditions and discrete Taylor vortex flow regimes, including laminar, wavy, and turbulent flows. While oxygen transport in Taylor vortex flows was significantly greater, and the available oxygen exceeded that consumed by murine fibroblasts in free suspension, the proportion of cells that remained viable decreased with increasing Reynolds number (101.8 < Rei < 1018), which we attribute to the action of fluid shear stresses on the cells as opposed to any limitation in mass transport. Nevertheless, the results of this study suggest that laminar Taylor-vortex flow regimes provide an effective means of maintaining the levels of oxygen transport required for long-term cell culture.
    Original languageEnglish
    Pages (from-to)766-773
    Number of pages7
    JournalBiotechnology and Bioengineering
    Volume89
    Issue number7
    DOIs
    Publication statusPublished - 2005

    Keywords

    • cells
    • tissue engineering
    • bioreactor
    • hydrodynamics
    • transport processes
    • bioengineering
    • biotechnology

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