Oxygen enrichment effects on protein oxidation, proteolytic activity and the energy status of submerged batch cultures of Aspergillus niger B1-D

Qiang Li, Linda M. Harvey, Brian McNeil

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Oxygen enrichment (sparging bioreactors with oxygen-enriched air) ameliorates oxygen limitation in batch cultures of Aspergillus niger B1-D, and although nutrient utilization rates for both the carbon source and the nitrogen source are higher in oxygen-enriched cultures, excess oxygen does not enhance specific growth rate, instead the 'extra' nutrient consumption is associated with defensive measures. Commencement of oxygen enrichment in early exponential phase leads to a transient rise in proteins showing oxidative damage (carbonylation), and to induction of enhanced proteolytic activity, which points to the antioxidant defense being temporarily overwhelmed, and that A. niger adapts to this oxidative environment by enhancing intracellular proteolytic activity to degrade damaged proteins which might otherwise accumulate under these conditions. Also the energy status of A. niger, reflected by intracellular ATP content, is found to be altered upon the commencement of oxygen enrichment and then reaches much lower levels than in the control. The reasons underlying this are discussed in the context of what is known regarding alternative respiration in industrial fungi.
LanguageEnglish
Pages238-243
Number of pages6
JournalProcess Biochemistry
Volume43
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Batch Cell Culture Techniques
Aspergillus niger
Aspergillus
Oxygen
Proteins
Oxidation
Nutrients
Carbonylation
Food
Adenosinetriphosphate
Bioreactors
Antioxidants
Fungi
Respiration
Nitrogen
Carbon
Adenosine Triphosphate
Air
Growth

Keywords

  • oxygen enrichment
  • Aspergillus niger
  • fermentation
  • protein carbonyl
  • proteolytic activity
  • ATP

Cite this

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abstract = "Oxygen enrichment (sparging bioreactors with oxygen-enriched air) ameliorates oxygen limitation in batch cultures of Aspergillus niger B1-D, and although nutrient utilization rates for both the carbon source and the nitrogen source are higher in oxygen-enriched cultures, excess oxygen does not enhance specific growth rate, instead the 'extra' nutrient consumption is associated with defensive measures. Commencement of oxygen enrichment in early exponential phase leads to a transient rise in proteins showing oxidative damage (carbonylation), and to induction of enhanced proteolytic activity, which points to the antioxidant defense being temporarily overwhelmed, and that A. niger adapts to this oxidative environment by enhancing intracellular proteolytic activity to degrade damaged proteins which might otherwise accumulate under these conditions. Also the energy status of A. niger, reflected by intracellular ATP content, is found to be altered upon the commencement of oxygen enrichment and then reaches much lower levels than in the control. The reasons underlying this are discussed in the context of what is known regarding alternative respiration in industrial fungi.",
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Oxygen enrichment effects on protein oxidation, proteolytic activity and the energy status of submerged batch cultures of Aspergillus niger B1-D. / Li, Qiang; Harvey, Linda M.; McNeil, Brian.

In: Process Biochemistry, Vol. 43, No. 3, 03.2008, p. 238-243.

Research output: Contribution to journalArticle

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AB - Oxygen enrichment (sparging bioreactors with oxygen-enriched air) ameliorates oxygen limitation in batch cultures of Aspergillus niger B1-D, and although nutrient utilization rates for both the carbon source and the nitrogen source are higher in oxygen-enriched cultures, excess oxygen does not enhance specific growth rate, instead the 'extra' nutrient consumption is associated with defensive measures. Commencement of oxygen enrichment in early exponential phase leads to a transient rise in proteins showing oxidative damage (carbonylation), and to induction of enhanced proteolytic activity, which points to the antioxidant defense being temporarily overwhelmed, and that A. niger adapts to this oxidative environment by enhancing intracellular proteolytic activity to degrade damaged proteins which might otherwise accumulate under these conditions. Also the energy status of A. niger, reflected by intracellular ATP content, is found to be altered upon the commencement of oxygen enrichment and then reaches much lower levels than in the control. The reasons underlying this are discussed in the context of what is known regarding alternative respiration in industrial fungi.

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