Over-expression of MAP kinase phosphatase-2 enhances adhesion molecule expression and protects against apoptosis in human endothelial cells

Mashael Al-Mutairi, Sameer Al-Harthi, Laurence Cadalbert, Robin Plevin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study we used adenovirus infection to overexpress the dual specific phosphatase, MAP kinase phosphatase-2 (MKP-2), in human umbilical vein endothelial cells and examined inflammatory protein expression and apoptosis, two key features of endothelial dysfunction in disease. We generated an adenoviral version of MKP-2 (Adv.MKP-2) and infected HUVECs for 40 h. TNF! stimulated MAP kinase phosphorylation and protein expression was measured by Western blotting. Cellular apoptosis was assayed by FACS. Infection with Adv.MKP-2 selectively abolished TNF!-mediated JNK activation and had little effect upon ERK or p38 MAP kinase. Adv.MKP-2 abrogated COX-2 expression whilst induction of the endothelial cell adhesion molecules ICAM and VCAM, two NF"B-dependent proteins, were not affected. However, when ICAM and VCAM expression was partly reduced by blockage of the NF"B pathway Adv.MKP-2 was able to reverse this inhibition. This correlated with enhanced TNF!-induced I"B! loss, a marker of NF"B activation. TNF! in combination with NF"B blockade also increased HUVEC apoptosis; this was significantly reversed by Adv.MKP-2. Protein markers of cellular damage and apoptosis, H2AX phosphorylation and caspase-3 cleavage, were also reversed by MKP-2 overexpression.
LanguageEnglish
Pages782-798
Number of pages17
JournalBritish Journal of Pharmacology
Volume161
Issue number4
DOIs
Publication statusPublished - 2010

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Mitogen-Activated Protein Kinase 1
Phosphoric Monoester Hydrolases
Endothelial Cells
Apoptosis
Phosphorylation
Adenoviridae Infections
Extracellular Signal-Regulated MAP Kinases
Human Umbilical Vein Endothelial Cells
Cell Adhesion Molecules
p38 Mitogen-Activated Protein Kinases
Caspase 3
Protein Kinases
Proteins
Western Blotting
Infection

Keywords

  • map kinase phosphatase-2
  • endothelial cell dysfunction
  • caspase
  • jnk
  • apoptosis

Cite this

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abstract = "In this study we used adenovirus infection to overexpress the dual specific phosphatase, MAP kinase phosphatase-2 (MKP-2), in human umbilical vein endothelial cells and examined inflammatory protein expression and apoptosis, two key features of endothelial dysfunction in disease. We generated an adenoviral version of MKP-2 (Adv.MKP-2) and infected HUVECs for 40 h. TNF! stimulated MAP kinase phosphorylation and protein expression was measured by Western blotting. Cellular apoptosis was assayed by FACS. Infection with Adv.MKP-2 selectively abolished TNF!-mediated JNK activation and had little effect upon ERK or p38 MAP kinase. Adv.MKP-2 abrogated COX-2 expression whilst induction of the endothelial cell adhesion molecules ICAM and VCAM, two NF{"}B-dependent proteins, were not affected. However, when ICAM and VCAM expression was partly reduced by blockage of the NF{"}B pathway Adv.MKP-2 was able to reverse this inhibition. This correlated with enhanced TNF!-induced I{"}B! loss, a marker of NF{"}B activation. TNF! in combination with NF{"}B blockade also increased HUVEC apoptosis; this was significantly reversed by Adv.MKP-2. Protein markers of cellular damage and apoptosis, H2AX phosphorylation and caspase-3 cleavage, were also reversed by MKP-2 overexpression.",
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Over-expression of MAP kinase phosphatase-2 enhances adhesion molecule expression and protects against apoptosis in human endothelial cells. / Al-Mutairi, Mashael; Al-Harthi, Sameer; Cadalbert, Laurence; Plevin, Robin.

In: British Journal of Pharmacology, Vol. 161, No. 4, 2010, p. 782-798.

Research output: Contribution to journalArticle

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