Oral delivery of tetanus toxoid using vesicles containing bile salts (bilosomes) induces significant systemic and mucosal immunity

J. Alexander, J.F.S. Mann, H.E. Scales, E. Shakir, K.C. Carter, A. Mullen, V.A. Ferro

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Protein antigens administered via the oral route are exposed to a hostile environment in the gastrointestinal tract, consisting of digestive enzymes and a range of pH (1-7.5). Using a delivery system can afford protection to entrapped components against degradation and permit delivery of antigen to the cells responsible for generating local and systemic immune responses. In this comparative study, mice were immunised orally with tetanus toxoid (40 or 200 μg dose/mouse, four doses in total) entrapped in non-ionic surfactant vesicles formulated with bile salts (bilosomes). The higher entrapped dose (BV-TT, 200 μg) induced IgG1 by study week 3 to similar levels to those observed with subcutaneous un-entrapped TT at the lower (<50 μg) dose. However, both bilosome formulations (BV-TT, low, and high doses), though not un-entrapped TT, caused a rise in the numbers of IgA positive plasma cells observed in the small intestine, primarily in the first 15 cm of the small intestine.
LanguageEnglish
Pages90-95
Number of pages5
JournalMethods
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 2006

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Mucosal Immunity
Tetanus Toxoid
Bile Acids and Salts
Small Intestine
Antigens
Nonionic surfactants
Plasma Cells
Surface-Active Agents
Immunoglobulin A
Gastrointestinal Tract
Immunoglobulin G
Plasmas
Degradation
Enzymes
Proteins

Keywords

  • oral delivery
  • vaccine
  • tetanus toxoid
  • antibody
  • immunohistochemistry

Cite this

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title = "Oral delivery of tetanus toxoid using vesicles containing bile salts (bilosomes) induces significant systemic and mucosal immunity",
abstract = "Protein antigens administered via the oral route are exposed to a hostile environment in the gastrointestinal tract, consisting of digestive enzymes and a range of pH (1-7.5). Using a delivery system can afford protection to entrapped components against degradation and permit delivery of antigen to the cells responsible for generating local and systemic immune responses. In this comparative study, mice were immunised orally with tetanus toxoid (40 or 200 μg dose/mouse, four doses in total) entrapped in non-ionic surfactant vesicles formulated with bile salts (bilosomes). The higher entrapped dose (BV-TT, 200 μg) induced IgG1 by study week 3 to similar levels to those observed with subcutaneous un-entrapped TT at the lower (<50 μg) dose. However, both bilosome formulations (BV-TT, low, and high doses), though not un-entrapped TT, caused a rise in the numbers of IgA positive plasma cells observed in the small intestine, primarily in the first 15 cm of the small intestine.",
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Oral delivery of tetanus toxoid using vesicles containing bile salts (bilosomes) induces significant systemic and mucosal immunity. / Alexander, J.; Mann, J.F.S.; Scales, H.E.; Shakir, E.; Carter, K.C.; Mullen, A.; Ferro, V.A.

In: Methods, Vol. 38, No. 2, 02.2006, p. 90-95.

Research output: Contribution to journalArticle

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