Abstract
| Original language | English |
|---|---|
| Article number | 25 |
| Number of pages | 15 |
| Journal | Frontiers in Neural Circuits |
| Volume | 9 |
| DOIs | |
| Publication status | Published - 29 May 2015 |
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Keywords
- optogenetics
- neurotechnology
- cortical layers
- neural circuit
- µLEDs
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Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe. / McAlinden, Niall; Gu, Erdan; Dawson, Martin D; Sakata, Shuzo; Mathieson, Keith.
In: Frontiers in Neural Circuits, Vol. 9, 25, 29.05.2015.Research output: Contribution to journal › Article
TY - JOUR
T1 - Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe
AU - McAlinden, Niall
AU - Gu, Erdan
AU - Dawson, Martin D
AU - Sakata, Shuzo
AU - Mathieson, Keith
PY - 2015/5/29
Y1 - 2015/5/29
N2 - Optogenetics has proven to be a revolutionary technology in neuroscience and has advanced continuously over the past decade. However, optical stimulation technologies for in vivo need to be developed to match the advances in genetics and biochemistry that have driven this field. In particular, conventional approaches for in vivo optical illumination have a limitation on the achievable spatio-temporal resolution. Here we utilize a sapphire-based microscale gallium nitride light-emitting diode (µLED) probe to activate neocortical neurons in vivo. The probes were designed to contain independently controllable multiple µLEDs, emitting at 450 nm wavelength with an irradiance of up to 2 W/mm2. Monte-Carlo stimulations predicted that optical stimulation using a µLED can modulate neural activity within a localized region. To validate this prediction, we tested this probe in the mouse neocortex that expressed channelrhodopsin-2 (ChR2) and compared the results with optical stimulation through a fiber at the cortical surface. We confirmed that both approaches reliably induced action potentials in cortical neurons and that the µLED probe evoked strong responses in deep neurons. Due to the possibility to integrate many optical stimulation sites onto a single shank, the µLED probe is thus a promising approach to control neurons locally in vivo.
AB - Optogenetics has proven to be a revolutionary technology in neuroscience and has advanced continuously over the past decade. However, optical stimulation technologies for in vivo need to be developed to match the advances in genetics and biochemistry that have driven this field. In particular, conventional approaches for in vivo optical illumination have a limitation on the achievable spatio-temporal resolution. Here we utilize a sapphire-based microscale gallium nitride light-emitting diode (µLED) probe to activate neocortical neurons in vivo. The probes were designed to contain independently controllable multiple µLEDs, emitting at 450 nm wavelength with an irradiance of up to 2 W/mm2. Monte-Carlo stimulations predicted that optical stimulation using a µLED can modulate neural activity within a localized region. To validate this prediction, we tested this probe in the mouse neocortex that expressed channelrhodopsin-2 (ChR2) and compared the results with optical stimulation through a fiber at the cortical surface. We confirmed that both approaches reliably induced action potentials in cortical neurons and that the µLED probe evoked strong responses in deep neurons. Due to the possibility to integrate many optical stimulation sites onto a single shank, the µLED probe is thus a promising approach to control neurons locally in vivo.
KW - optogenetics
KW - neurotechnology
KW - cortical layers
KW - neural circuit
KW - µLEDs
UR - http://www.frontiersin.org/neural_circuits/10.3389/fncir.2015.00025/abstract
U2 - 10.3389/fncir.2015.00025
DO - 10.3389/fncir.2015.00025
M3 - Article
VL - 9
JO - Frontiers in Neural Circuits
JF - Frontiers in Neural Circuits
SN - 1662-5110
M1 - 25
ER -