Projects per year
Optogenetics has proven to be a revolutionary technology in neuroscience and has advanced continuously over the past decade. However, optical stimulation technologies for in vivo need to be developed to match the advances in genetics and biochemistry that have driven this field. In particular, conventional approaches for in vivo optical illumination have a limitation on the achievable spatio-temporal resolution. Here we utilize a sapphire-based microscale gallium nitride light-emitting diode (µLED) probe to activate neocortical neurons in vivo. The probes were designed to contain independently controllable multiple µLEDs, emitting at 450 nm wavelength with an irradiance of up to 2 W/mm2. Monte-Carlo stimulations predicted that optical stimulation using a µLED can modulate neural activity within a localized region. To validate this prediction, we tested this probe in the mouse neocortex that expressed channelrhodopsin-2 (ChR2) and compared the results with optical stimulation through a fiber at the cortical surface. We confirmed that both approaches reliably induced action potentials in cortical neurons and that the µLED probe evoked strong responses in deep neurons. Due to the possibility to integrate many optical stimulation sites onto a single shank, the µLED probe is thus a promising approach to control neurons locally in vivo.
|Number of pages||15|
|Journal||Frontiers in Neural Circuits|
|Publication status||Published - 29 May 2015|
- cortical layers
- neural circuit
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- 3 Finished
State-dependent and cell-type-specific information processing in auditory cortex
BBSRC (Biotech & Biological Sciences Research Council)
19/08/13 → 18/08/16
Pathological ensembles in the auditory thalamocortical circuit following acoustic trauma
MRC (Medical Research Council)
1/12/12 → 31/05/14
SU2P: Stanford-Scotland Photonics Innovation Collaboration (Science Bridges)
Ferguson, A., Ackemann, T., Burns, D., Dawson, M., McConnell, G. & Riis, E.
EPSRC (Engineering and Physical Sciences Research Council)
1/09/09 → 28/02/13
Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe
McAlinden, N. (Creator), Sakata, S. (Creator) & Mathieson, K. (Contributor), University of Strathclyde, 2015