Abstract
A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.
Original language | English |
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Pages (from-to) | 1372-1380 |
Number of pages | 9 |
Journal | Optics Express |
Volume | 22 |
Issue number | 2 |
DOIs | |
Publication status | Published - 27 Jan 2014 |
Keywords
- optoelectronic tweezers
- cell manipulation
- gallium nitride
- micro-LED arrays
- compact optoelectronic tweezers system
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Data for "Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells"
Jeorrett, A. H. (Creator), Neale, S. L. (Creator), Massoubre, D. (Creator), Gu, E. (Contributor), Henderson, R. K. (Creator), Millington, O. (Creator), Mathieson, K. (Creator) & Dawson, M. D. (Creator), figshare, 21 Jun 2023
DOI: 10.6084/m9.figshare.c.3765128.v1
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