Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Abigail H Jeorrett, Steven L Neale, David Massoubre, Erdan Gu, Robert K Henderson, Owain Millington, Keith Mathieson, Martin D Dawson

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Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

Original languageEnglish
Pages (from-to)1372-1380
Number of pages9
JournalOptics Express
Volume22
Issue number2
DOIs
Publication statusPublished - 27 Jan 2014

Keywords

  • optoelectronic tweezers
  • cell manipulation
  • gallium nitride
  • micro-LED arrays
  • compact optoelectronic tweezers system

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