Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Abigail H Jeorrett, Steven L Neale, David Massoubre, Erdan Gu, Robert K Henderson, Owain Millington, Keith Mathieson, Martin D Dawson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

LanguageEnglish
Pages1372-1380
Number of pages9
JournalOptics Express
Volume22
Issue number2
DOIs
Publication statusPublished - 27 Jan 2014

Fingerprint

manipulators
fluorescence
light emitting diodes
cells
traps
gallium nitrides
lymphocytes
optoelectronic devices
CMOS
dyes
trapping
interactions
profiles
wavelengths

Keywords

  • optoelectronic tweezers
  • cell manipulation
  • gallium nitride
  • micro-LED arrays
  • compact optoelectronic tweezers system

Cite this

Jeorrett, Abigail H ; Neale, Steven L ; Massoubre, David ; Gu, Erdan ; Henderson, Robert K ; Millington, Owain ; Mathieson, Keith ; Dawson, Martin D. / Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells. In: Optics Express. 2014 ; Vol. 22, No. 2. pp. 1372-1380.
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abstract = "A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.",
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Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells. / Jeorrett, Abigail H; Neale, Steven L; Massoubre, David; Gu, Erdan; Henderson, Robert K; Millington, Owain; Mathieson, Keith; Dawson, Martin D.

In: Optics Express, Vol. 22, No. 2, 27.01.2014, p. 1372-1380.

Research output: Contribution to journalArticle

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