Abstract
A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.
Original language | English |
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Pages (from-to) | 1372-1380 |
Number of pages | 9 |
Journal | Optics Express |
Volume | 22 |
Issue number | 2 |
DOIs | |
Publication status | Published - 27 Jan 2014 |
Keywords
- optoelectronic tweezers
- cell manipulation
- gallium nitride
- micro-LED arrays
- compact optoelectronic tweezers system