Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Abigail H Jeorrett; Steven L Neale; David Massoubre; Erdan Gu; Robert K Henderson; Owain Millington; Keith Mathieson; Martin D Dawson

doi:10.1364/OE.22.001372

Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Abigail H Jeorrett, Steven L Neale, David Massoubre, Erdan Gu, Robert K Henderson, Owain Millington, Keith Mathieson, Martin D Dawson

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Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

Original language	English
Pages (from-to)	1372-1380
Number of pages	9
Journal	Optics Express
Volume	22
Issue number	2
DOIs	https://doi.org/10.1364/OE.22.001372
Publication status	Published - 27 Jan 2014

Keywords

optoelectronic tweezers
cell manipulation
gallium nitride
micro-LED arrays
compact optoelectronic tweezers system

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10.1364/OE.22.001372

oe-22-2-1372Final published version, 1.04 MBLicence: CC BY 4.0

Cite this

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title = "Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells",

abstract = "A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.",

keywords = "optoelectronic tweezers, cell manipulation, gallium nitride, micro-LED arrays, compact optoelectronic tweezers system",

author = "Jeorrett, {Abigail H} and Neale, {Steven L} and David Massoubre and Erdan Gu and Henderson, {Robert K} and Owain Millington and Keith Mathieson and Dawson, {Martin D}",

note = "This paper demonstrates a novel and compact {\textquoteleft}lab-on-a-chip{\textquoteright} system for controllably manipulating single cells, based on light-activated dielectrophoresis traps within an optoelectronic tweezers device. The device is enabled by CMOS-controlled gallium nitride LED arrays and both these and the trapping system were developed and evaluated at Strathclyde. The capability of the system is demonstrated for controlled interaction of single T-lymphocytes and dendritic cells. Professor Dawson conceived this area of research and provided overall supervision and direction to the work. The paper has been cited 10 times to date (Google Scholar).",

year = "2014",

month = jan,

day = "27",

doi = "10.1364/OE.22.001372",

language = "English",

volume = "22",

pages = "1372--1380",

journal = "Optics Express",

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T1 - Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

AU - Jeorrett, Abigail H

AU - Neale, Steven L

AU - Massoubre, David

AU - Gu, Erdan

AU - Henderson, Robert K

AU - Millington, Owain

AU - Mathieson, Keith

AU - Dawson, Martin D

N1 - This paper demonstrates a novel and compact ‘lab-on-a-chip’ system for controllably manipulating single cells, based on light-activated dielectrophoresis traps within an optoelectronic tweezers device. The device is enabled by CMOS-controlled gallium nitride LED arrays and both these and the trapping system were developed and evaluated at Strathclyde. The capability of the system is demonstrated for controlled interaction of single T-lymphocytes and dendritic cells. Professor Dawson conceived this area of research and provided overall supervision and direction to the work. The paper has been cited 10 times to date (Google Scholar).

PY - 2014/1/27

Y1 - 2014/1/27

N2 - A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

AB - A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

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Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

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Martin Dawson

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Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

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Martin Dawson

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