Optimisation of a lipid based oral delivery system containing A/Panama influenza haemagglutinin

J.F.S. Mann, V.A. Ferro, A. Mullen, L. Tetley, M. Mullen, K.C. Carter, J. Alexander, W.H. Stimson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Vaccine antigens administered by the oral route are often degraded by gastric secretions during gastrointestinal transit. This necessitates larger and more frequent doses of antigen for vaccination. A delivery system, which overcomes this, is a lipid vesicle containing bile salts (bilosome), which prevents antigen degradation and enhances mucosal penetration. The effect of bilosome formulation modification on vaccine transit efficacy across the mucosa was determined. Specific antibody levels were assessed by end-point titre ELISA and the subclasses determined. Significant IgG1 titres were induced when the protein loading was doubled from 15 to 30 μg (P=0.009) and was equivalent to antigen administration by the subcutaneous route. No IgG2a was induced, indicating the generation of a TH2 response. Significant mucosal IgA levels were also observed with this treatment group (P=0.05).
LanguageEnglish
Pages2425-2429
Number of pages4
JournalVaccine
Volume22
Issue number19
DOIs
Publication statusPublished - 2004

Fingerprint

Panama
Hemagglutinins
hemagglutinins
influenza
Human Influenza
mouth
antigens
Lipids
Antigens
lipids
Vaccines
Gastrointestinal Transit
vaccines
gastrointestinal transit
bile salts
Bile Acids and Salts
Immunoglobulin A
mucosa
Stomach
Mucous Membrane

Keywords

  • influenza
  • oral immunisation
  • lipid vesicles
  • immunology
  • pharmacology

Cite this

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abstract = "Vaccine antigens administered by the oral route are often degraded by gastric secretions during gastrointestinal transit. This necessitates larger and more frequent doses of antigen for vaccination. A delivery system, which overcomes this, is a lipid vesicle containing bile salts (bilosome), which prevents antigen degradation and enhances mucosal penetration. The effect of bilosome formulation modification on vaccine transit efficacy across the mucosa was determined. Specific antibody levels were assessed by end-point titre ELISA and the subclasses determined. Significant IgG1 titres were induced when the protein loading was doubled from 15 to 30 μg (P=0.009) and was equivalent to antigen administration by the subcutaneous route. No IgG2a was induced, indicating the generation of a TH2 response. Significant mucosal IgA levels were also observed with this treatment group (P=0.05).",
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Optimisation of a lipid based oral delivery system containing A/Panama influenza haemagglutinin. / Mann, J.F.S.; Ferro, V.A.; Mullen, A.; Tetley, L.; Mullen, M.; Carter, K.C.; Alexander, J.; Stimson, W.H.

In: Vaccine, Vol. 22, No. 19, 2004, p. 2425-2429.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Optimisation of a lipid based oral delivery system containing A/Panama influenza haemagglutinin

AU - Mann, J.F.S.

AU - Ferro, V.A.

AU - Mullen, A.

AU - Tetley, L.

AU - Mullen, M.

AU - Carter, K.C.

AU - Alexander, J.

AU - Stimson, W.H.

PY - 2004

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AB - Vaccine antigens administered by the oral route are often degraded by gastric secretions during gastrointestinal transit. This necessitates larger and more frequent doses of antigen for vaccination. A delivery system, which overcomes this, is a lipid vesicle containing bile salts (bilosome), which prevents antigen degradation and enhances mucosal penetration. The effect of bilosome formulation modification on vaccine transit efficacy across the mucosa was determined. Specific antibody levels were assessed by end-point titre ELISA and the subclasses determined. Significant IgG1 titres were induced when the protein loading was doubled from 15 to 30 μg (P=0.009) and was equivalent to antigen administration by the subcutaneous route. No IgG2a was induced, indicating the generation of a TH2 response. Significant mucosal IgA levels were also observed with this treatment group (P=0.05).

KW - influenza

KW - oral immunisation

KW - lipid vesicles

KW - immunology

KW - pharmacology

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