Optically detected magnetic resonance of nanodiamond using widefield detection

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Research into the crystalline defect in diamond is well documented throughout literature as a means to acquire magnetic field and thermal measurements, the process of acquiring this data from the Nitrogen Vacancy (NV-) center is known as Optically Detected Magnetic Resonance (ODMR). When illuminated with green light the NV becomes optically active and emits in the near-infared, applying microwave field scanning around the resonant frequency (2.875GHz) causes a decrease in fluorescent output as it decays via the spin singlet state, known as the "dark decay path", shifts in this frequency or Zeeman splitting allows measurements of the thermal or magnetic fields respectively [1].
Nanodiamond has gained notoriety as a bio-compatible and stable fluorescent emitter since there is no fluorescent decay. This makes the nanodiamond an excellent choice as a marker for cellular biological sensing and opens the door to very sensitive measurements with excellent spatial information [2]. Due to our want to image nanodiamond in vivo we have custom built a system for widefield Total Internal Reflection Fluorescence (TIRF) microscopy and Epifluorescent microscopy, to allow the dual functionality of excitation of NV- with green illumination using TIRF combined with the excitation of fluorophores using Epifluorescence which reduces the light exposure to cells and fluorophores during the imaging process.
[1] L. Rodin et al., "Magnetometry with nitrogen-vacancy defects in diamond," Rep. Prog. Phys., vol. 77, 2014.
[2] H. Yukawa et al., "A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature," Nanoscale Adv., vol. 2, 2020.
Original languageEnglish
Number of pages1
Publication statusPublished - 29 Nov 2023
EventSymbiosis: Healthcare Biosensing and Bioimaging Exchange - University of Glasgow, Glasgow
Duration: 29 Nov 202329 Nov 2023




  • optically detected magnetic resonance
  • fluorescent nanodiamond
  • epifluorescence imaging


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