Optical trapping and manipulation of live T cells with a low numerical aperture lens

J. Harris, G. McConnell

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An optical manipulation system that employs both optical andtemperature gradients to simultaneously enable trapping, manipulating and imaging of live cells with a low magnification, low numerical aperture objective lens (10x/0.4 N.A.) is reported. This approach negatesthe requirement for a high N.A. lens used in traditional optical trapping.Our system comprised a dual scanning system and two independent lasers which provided the ability to move the trapping spot independently of the confocal imaging process in close to real-time and without pre-programming. To demonstrate the efficacy of this innovativemethod, trapping and manipulation of live T cells was simultaneously performed over a field of view exceeding 1 mm2 for extended periods without compromising cell viability.
Original languageEnglish
Pages (from-to)14036-14043
Number of pages8
JournalOptics Express
Volume16
Issue number18
DOIs
Publication statusPublished - 1 Sep 2008

Keywords

  • optical trapping
  • manipulation
  • live T cells
  • low numerical aperture lens

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