Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

V. Poher, H. Zhang, G.T. Kennedy, C. Griffin, S. Oddos, E. Gu, D.S. Elson, J.M. Girkin, P.M.W. French, M.D. Dawson, M.A. Neil

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Abstract

We describe an optical sectioning microscopy system with no moving parts based on a micro-structured stripe-array light emitting diode (LED). By projecting arbitrary line or grid patterns onto the object, we are able to implement a variety of optical sectioning microscopy techniques such as grid-projection structured illumination and line scanning confocal microscopy, switching from one imaging technique to another without modifying the microscope setup. The micro-structured LED and driver are detailed and depth discrimination capabilities are measured and calculated.
Original languageEnglish
Pages (from-to)11196 -11206
JournalOptics Express
Volume15
Issue number18
DOIs
Publication statusPublished - 21 Aug 2007

Keywords

  • confocal microscopy
  • light-emitting diodes
  • fluorescence microscopy
  • microstructure devices

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