Optical physics enables advances in multiphoton imaging

J.M. Girkin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Since the initial images were taken using a multiphoton imaging technique the method has rapidly established itself as the preferred method for imaging deeply into biological samples with micron resolution in three dimensions. Multiphoton imaging has thus enabled researchers in the life sciences to undertake studies that had previously been believed to be impossible without significantly perturbing the sample. Many of these experiments have only been realized due to close cooperation between optical physicists, from a range of disciplines, and the biomedical researchers. This paper will provide a general review of the current state of the field demonstrating how the various aspects of the physics development have brought the multiphoton technique to its current position at the forefront of biological microscopy.
LanguageEnglish
PagesR250-R258
JournalJournal of Physics D: Applied Physics
Volume36
Issue number14
DOIs
Publication statusPublished - Jul 2003

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Physics
Imaging techniques
life sciences
physics
imaging techniques
microscopy
Microscopic examination
Experiments

Keywords

  • scanning fluorescence microscopy
  • surface-emitting laser
  • 2-photon excitation
  • mode-locking
  • high-power
  • pulse
  • dispersion

Cite this

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Optical physics enables advances in multiphoton imaging. / Girkin, J.M.

In: Journal of Physics D: Applied Physics, Vol. 36, No. 14, 07.2003, p. R250-R258.

Research output: Contribution to journalArticle

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