Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation

J.F.A. Fernandes, M. McAlpine, P.J. Halling

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The operational stability of cross-linked crystals (CLECs) of subtilisin Carlsberg in organic media is compared between repeated batch and continuous flow operation. The study was designed to create similar conditions in the two modes, although these can never be identical in all respects. The biocatalyst inactivates rapidly during the first two batches of 2 h each, losing 50% or more of its activity. The inactivation becomes slower over the following longer batches of 24 h. This pattern of rapid and slower phases of inactivation is similar to that observed in continuous reactors. However, in both acetonitrile and tetrahydrofuran, inactivation is somewhat faster over the first 4h of continuous operation than in the equivalent batch mode.
LanguageEnglish
Pages11-15
Number of pages4
JournalBiochemical Engineering Journal
Volume24
Issue number1
DOIs
Publication statusPublished - 15 May 2005

Fingerprint

Subtilisins
Subtilisin
Biocatalysts
Acetonitrile
Organic solvents
Crystals
Enzymes
tetrahydrofuran
acetonitrile

Keywords

  • cross-linked crystals
  • CLECs
  • continuous flow operation

Cite this

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abstract = "The operational stability of cross-linked crystals (CLECs) of subtilisin Carlsberg in organic media is compared between repeated batch and continuous flow operation. The study was designed to create similar conditions in the two modes, although these can never be identical in all respects. The biocatalyst inactivates rapidly during the first two batches of 2 h each, losing 50{\%} or more of its activity. The inactivation becomes slower over the following longer batches of 24 h. This pattern of rapid and slower phases of inactivation is similar to that observed in continuous reactors. However, in both acetonitrile and tetrahydrofuran, inactivation is somewhat faster over the first 4h of continuous operation than in the equivalent batch mode.",
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Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation. / Fernandes, J.F.A.; McAlpine, M.; Halling, P.J.

In: Biochemical Engineering Journal, Vol. 24, No. 1, 15.05.2005, p. 11-15.

Research output: Contribution to journalArticle

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