On the specificity of reactions catalysed by the antibody H11

Abedawn I. Khalaf, Sabin Linaza, Andrew R. Pitt, William H. Stimson, Colin J. Suckling

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The substrate specificity and the stereochem. course of the reactions catalyzed by the antibody H11 (which was raised to a protein conjugated deriv. of the adduct of 1-acetoxy-buta-1,3-diene 1) have been investigated. The antibody shows high selectivity for acetoxybutadiene which it hydrolyzes to the corresponding dienol, the major diene component of the cycloaddn. reactions obsd. However, it tolerates a range of N-alkylmaleimides. The stereochem. course of cycloaddn. is shown to produce a significant enantiomeric excess of the 3aR,4S,7aR-endo-diastereoisomer by anal. with Mosher's ester derivs. This study also revealed that H11 is capable of slowly catalyzing the hydrolysis of N-alkylmaleimide substrates. The implications for the mechanism of action of H11 are discussed. [on SciFinder(R)]
Original languageEnglish
Pages (from-to)489-495
Number of pages7
Issue number3
Publication statusPublished - 14 Jan 2000


  • antibody H11
  • acetoxybutadiene
  • Mosher's ester
  • pure and applied chemistry


Dive into the research topics of 'On the specificity of reactions catalysed by the antibody H11'. Together they form a unique fingerprint.

Cite this