On the blockade of acetylcholine release at mouse motor nerve terminals by β-bungarotoxin and crotoxin

E. G. Rowan, K. E. Pemberton, A. L. Harvey

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

β-Bungarotoxin and crotoxin are phospholipase A2 neurotoxins, which block irreversibly the evoked release of acetylcholine from motor nerve terminals of mouse triangularis sterni preperations. Extracellular recording of nerve terminal action potentials reveal that inhibition of transmitter release is not associated with failure of the action potential to invade nerve terminals. When evoked transmitter release (measured as intracellularly recorded endplate potentials) was blocked by β-bungarotoxin, spontaneous acetylcholine release was stimulated as in control experiments by K+-induced depolarization and by the Ca2+-ionophore A23187. The site of action of the toxins remains to be elucidated but would appear to be associated with the coupling of action potential induced-depolarization to the release mechanism, rather than with the release mechanism itself.

LanguageEnglish
Pages301-304
Number of pages4
JournalBritish Journal of Pharmacology
Volume100
Issue number2
DOIs
Publication statusPublished - 1 Jan 1990

Fingerprint

Crotoxin
Bungarotoxins
Action Potentials
Acetylcholine
Phospholipases A2
Ionophores
Calcimycin
Neurotoxins

Keywords

  • beta bungarotoxin
  • calcimycin
  • crotoxin
  • potassium ion
  • snake venom
  • tubocurarine chloride
  • acetylcholine release
  • action potential
  • nerve ending
  • neuromuscular synapse

Cite this

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On the blockade of acetylcholine release at mouse motor nerve terminals by β-bungarotoxin and crotoxin. / Rowan, E. G.; Pemberton, K. E.; Harvey, A. L.

In: British Journal of Pharmacology, Vol. 100, No. 2, 01.01.1990, p. 301-304.

Research output: Contribution to journalArticle

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