On-chip optical stimulation and electrical recording from cells

A. Yakushenko, Zheng Gong, V. Maybeck, B. Hofmann, Erdan Gu, Martin Dawson, Andreas Offenhäusser, Bernhard Wolfrum

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.
LanguageEnglish
Article number111402
JournalJournal of Biomedical Optics
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 2013

Fingerprint

stimulation
recording
chips
Microelectrodes
cells
Cell culture
Light emitting diodes
Light sources
Calcium
Experiments
Cells
Proteins
Imaging techniques
cultured cells
calcium
light sources
light emitting diodes
proteins

Keywords

  • optoelectrical devices
  • light-emitting diodes
  • planar microelectrode array

Cite this

Yakushenko, A. ; Gong, Zheng ; Maybeck, V. ; Hofmann, B. ; Gu, Erdan ; Dawson, Martin ; Offenhäusser, Andreas ; Wolfrum, Bernhard. / On-chip optical stimulation and electrical recording from cells. In: Journal of Biomedical Optics. 2013 ; Vol. 18, No. 11.
@article{3b7ec871c8674863b7946cb588e37e86,
title = "On-chip optical stimulation and electrical recording from cells",
abstract = "We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.",
keywords = "optoelectrical devices, light-emitting diodes, planar microelectrode array",
author = "A. Yakushenko and Zheng Gong and V. Maybeck and B. Hofmann and Erdan Gu and Martin Dawson and Andreas Offenh{\"a}usser and Bernhard Wolfrum",
year = "2013",
month = "11",
doi = "10.1117/1.JBO.18.11.111402",
language = "English",
volume = "18",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
number = "11",

}

Yakushenko, A, Gong, Z, Maybeck, V, Hofmann, B, Gu, E, Dawson, M, Offenhäusser, A & Wolfrum, B 2013, 'On-chip optical stimulation and electrical recording from cells' Journal of Biomedical Optics, vol. 18, no. 11, 111402. https://doi.org/10.1117/1.JBO.18.11.111402

On-chip optical stimulation and electrical recording from cells. / Yakushenko, A.; Gong, Zheng; Maybeck, V.; Hofmann, B.; Gu, Erdan; Dawson, Martin; Offenhäusser, Andreas; Wolfrum, Bernhard.

In: Journal of Biomedical Optics, Vol. 18, No. 11, 111402, 11.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - On-chip optical stimulation and electrical recording from cells

AU - Yakushenko, A.

AU - Gong, Zheng

AU - Maybeck, V.

AU - Hofmann, B.

AU - Gu, Erdan

AU - Dawson, Martin

AU - Offenhäusser, Andreas

AU - Wolfrum, Bernhard

PY - 2013/11

Y1 - 2013/11

N2 - We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.

AB - We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.

KW - optoelectrical devices

KW - light-emitting diodes

KW - planar microelectrode array

U2 - 10.1117/1.JBO.18.11.111402

DO - 10.1117/1.JBO.18.11.111402

M3 - Article

VL - 18

JO - Journal of Biomedical Optics

T2 - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 11

M1 - 111402

ER -