On-chip immunoprecipitation for protein purification

Mairi E. Sandison, Sarah A. Cumming, Walter Kolch, Andrew R. Pitt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation (µIP) method for the on-chip purification of proteins from complex mixtures is described. A 4 µl PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the µIP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised µIP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate.

Original languageEnglish
Pages (from-to)2805-2813
Number of pages9
JournalLab on a Chip
Volume10
Issue number20
Early online date17 Aug 2010
DOIs
Publication statusPublished - 21 Oct 2010

Keywords

  • equipment design
  • equipment failure analysis
  • immunoprecipitation
  • microfluidic analytical techniques
  • proteins

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