On-chip high-speed sorting of micron-sized particles for high-throughput analysis

D. Holmes, M.E. Sandison, N.G. Green, H. Morgan

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A new design of particle sorting chip is presented. The device employs a dielectrophoretic gate that deflects particles into one of two microfluidic channels at high speed. The device operates by focussing particles into the central streamline of the main flow channel using dielectrophoretic focussing. At the sorting junction (T- or Y-junction) two sets of electrodes produce a small dielectrophoretic force that pushes the particle into one or other of the outlet channels, where they are carried under the pressure-driven fluid flow to the outlet. For a 40 microm wide and high channel, it is shown that 6 microm diameter particles can be deflected at a rate of 300/s. The principle of a fully automated sorting device is demonstrated by separating fluorescent from non-fluorescent latex beads.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalIEE Proceedings Nanobiotechnology
Volume152
Issue number4
DOIs
Publication statusPublished - Aug 2005

Keywords

  • cell separation
  • electrophoresis
  • equipment design
  • equipment failure analysis
  • flow cytometry
  • flow injection analysis
  • microfluidic analytical techniques
  • microspheres
  • nanotechnology
  • particle size
  • ultrafiltration

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