On-chip high-speed sorting of micron-sized particles for high-throughput analysis

D. Holmes, M.E. Sandison, N.G. Green, H. Morgan

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A new design of particle sorting chip is presented. The device employs a dielectrophoretic gate that deflects particles into one of two microfluidic channels at high speed. The device operates by focussing particles into the central streamline of the main flow channel using dielectrophoretic focussing. At the sorting junction (T- or Y-junction) two sets of electrodes produce a small dielectrophoretic force that pushes the particle into one or other of the outlet channels, where they are carried under the pressure-driven fluid flow to the outlet. For a 40 microm wide and high channel, it is shown that 6 microm diameter particles can be deflected at a rate of 300/s. The principle of a fully automated sorting device is demonstrated by separating fluorescent from non-fluorescent latex beads.

LanguageEnglish
Pages129-135
Number of pages7
JournalIEE Proceedings Nanobiotechnology
Volume152
Issue number4
DOIs
Publication statusPublished - Aug 2005

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Sorting
Throughput
Latex
Channel flow
Latexes
Microfluidics
Flow of fluids
Electrodes

Keywords

  • cell separation
  • electrophoresis
  • equipment design
  • equipment failure analysis
  • flow cytometry
  • flow injection analysis
  • microfluidic analytical techniques
  • microspheres
  • nanotechnology
  • particle size
  • ultrafiltration

Cite this

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abstract = "A new design of particle sorting chip is presented. The device employs a dielectrophoretic gate that deflects particles into one of two microfluidic channels at high speed. The device operates by focussing particles into the central streamline of the main flow channel using dielectrophoretic focussing. At the sorting junction (T- or Y-junction) two sets of electrodes produce a small dielectrophoretic force that pushes the particle into one or other of the outlet channels, where they are carried under the pressure-driven fluid flow to the outlet. For a 40 microm wide and high channel, it is shown that 6 microm diameter particles can be deflected at a rate of 300/s. The principle of a fully automated sorting device is demonstrated by separating fluorescent from non-fluorescent latex beads.",
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On-chip high-speed sorting of micron-sized particles for high-throughput analysis. / Holmes, D.; Sandison, M.E.; Green, N.G.; Morgan, H.

In: IEE Proceedings Nanobiotechnology, Vol. 152, No. 4, 08.2005, p. 129-135.

Research output: Contribution to journalArticle

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AU - Holmes, D.

AU - Sandison, M.E.

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AU - Morgan, H.

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KW - cell separation

KW - electrophoresis

KW - equipment design

KW - equipment failure analysis

KW - flow cytometry

KW - flow injection analysis

KW - microfluidic analytical techniques

KW - microspheres

KW - nanotechnology

KW - particle size

KW - ultrafiltration

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